Abstract: A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes ?-D-galactosidase and ?-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production. A second filter means is used to separate the cultured bacteria from the broth. A lysing agent is exposed to the bacteria on the second filter and incubated with a chemiluminogenic substrate of the enzyme to produce a chemiluminescent product. Light emission is initiated from the second filter means and the emitted light is detected or measured directly from the second filter means using a luminometer adapted to receive the second filter means.
Type:
Grant
Filed:
October 17, 2002
Date of Patent:
September 2, 2008
Assignee:
Conestoga - Rovers and Associates Limited
Inventors:
Garry A. Palmateer, Katarina D. M. Pintar, Michele I. Van Dyke
Abstract: A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes ?-D-galactosidase and ?-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production. A second filter means is used to separate the cultured bacteria from the broth. A lysing agent is exposed to the bacteria on the second filter and incubated with a chemiluminogenic substrate of the enzyme to produce a chemiluminescent product. Light emission is initiated from the second filter means and the emitted light is detected or measured directly from the second filter means using a luminometer adapted to receive the second filter means.
Type:
Application
Filed:
October 17, 2002
Publication date:
June 22, 2006
Applicant:
Conestoga-Rovers & Associates Limited
Inventors:
Garry Palmateer, Katarina Pintar, Michele Van Dyke