Abstract: ADMA is determined in a physiological sample by first removing interfering components: proteins by precipitation; and amines and citrulline, by means of a cation exchange column, followed by enzymatic hydrolysis of the ADMA to citrulline with DDAH. The citrulline is then determined spectrophotometrically.
Type:
Grant
Filed:
February 1, 2000
Date of Patent:
March 19, 2002
Assignees:
Cooke Pharma, Panorama Research, Inc.
Inventors:
Robert F. Balint, Mitchell Wayne Mutz, John P. Cooke
Abstract: Health bars are provided having high dosages of at least one of the amino acids L-arginine and L-lysine in conjunction with fruit solids and paste, protein and carbohydrates. A number of different fruit sources may be used, grapes finding particular use, and soy being a useful source of proteins. The bars are made by preparing a syrup at an elevated temperature, adding a fruit paste and cooling, followed by the addition of minor ingredients and mixing, the amino acids and a portion of the protein and mixing, followed by the remaining ingredients and formation as a bar.
Type:
Grant
Filed:
May 19, 1998
Date of Patent:
May 16, 2000
Assignee:
Cooke Pharma
Inventors:
Andrew J. Maxwell, John P. Cooke, Thomas Martin Radke