Abstract: A blood fluid composition for use in a complement-mediated cell lysis system. The composition includes a blood fluid, which may be either a serum source of complement, analyte-containing serum, or both, and lipid vesicles capable of reducing the extent of non-specific cell lysis produced when the blood fluid is added to lysable target cells in the system. The vesicles are present in an amount which increases the ratio of ligand-specific to non-specific cell lysis in the system at least 2-fold and preferably 4-fold or more over that achievable in the system in the absence of the vesicles.
Type:
Grant
Filed:
February 23, 1984
Date of Patent:
May 17, 1988
Assignee:
Cooper-Lipotech Partnership
Inventors:
Hans Schreier, Francis J. Martin, Viola T. Kung, Francis C. Szoka