Abstract: The methods and systems disclosed herein may relate to a testing cartridge. A system may include, a test cartridge comprising: a body comprising an upper component and a lower component, an inner cavity at least partially enclosed by the body, a sample port disposed in the upper component, a structural member disposed within the upper component relative to the sample port capable of controlling the contact between a sample and the sample pad, an assay coupled to the lower component, wherein the assay comprises a sample pad, and a membrane coupled to a portion of the structural member capable of pretreating the sample.

Abstract: Methods and systems directed towards a single use nasopharyngeal aspirate collection device comprise a reservoir capable of contracting and expanding in a lineal motion at least one time, a nozzle comprising an at least one orifice at a distal end of the nozzle, a fastener capable of coupling a proximal end of the reservoir with a proximal end of the nozzle, a guard disposed about a portion of an outer wall of the reservoir adjacent to the proximal end of the reservoir, and an internal cavity extending from the reservoir through the nozzle.