Abstract: The present invention relates to methods and devices for detecting one or more analytes in a biological sample, preferably a clean liquid sample. This invention in particular relates to improved rapid tests such as “dipsticks”, “lateral flow” devices and “flow-through” devices. The invention in particular relates to oligochromatographic devices that make use of a peptide- or hapten-coupled oligonucleotide and a reagent specifically recognizing the hapten or peptide and a conjugated probe that hybridizes specifically to a target sequence. It allows to detect specifically the presence of a polynucleotides directly or after molecular amplification steps with the use of a specific genuine internal control and a chromatographic control.

Abstract: The present invention relates to methods and devices for detecting one or more analytes in a biological sample, preferably a clean liquid sample. This invention in particular relates to improved rapid tests such as “dipsticks”, “lateral flow” devices and “flow-through” devices. The invention in particular relates to oligochromatographic devices that make use of a peptide- or hapten-coupled oligonucleotide and a reagent specifically recognizing the hapten or peptide and a conjugated probe that hybridizes specifically to a target sequence. It allows to detect specifically the presence of a polynucleotides directly or after molecular amplification steps with the use of a specific genuine internal control and a chromatographic control.

Abstract: The present invention relates to methods and devices for detecting one or more analytes or analyte products in a biological sample. This invention in particular relates to improved rapid tests such as “dipsticks” and “lateral flow” devices. The invention in particular relates to chromatographic device made of two active sides, in order to allow multiplex detections, quantitative or semi-quantitative detections, use of multiple sizes and kinds of particles or microspheres, use of different nitrocellulose porosities, use of different kinds of membranes. The devices described in this invention allow to detect or identify various biologicals or chemicals with one manipulation.