Abstract: The purpose of the present invention is to provide a method of purification and preparation of cultured corneal endothelial cells, and in particular, to provide cell surface markers for use in corneal endothelial cells not including transformed cells. Provided are cell markers for distinguishing normal cells and transformed cells, in particular normal and transformed corneal endothelium cells. These cell markers relate to specific cell surface markers, for example, to a normal corneal endothelial surface marker such as CD166, and a transformed cell surface marker such as CD73. By using the transformed cell surface marker such as CD73 to remove transformed cells by sorting, it becomes possible to improve purity of a normal cultured corneal endothelium. By using normal corneal endothelial surface marker such as CD166, or by combined use with the transformed cell surface marker, it becomes possible to provide a means for verifying the purity of a prepared corneal endothelium.

Abstract: A dissection system for corneal transplants includes a housing including a contact side to be positioned against a cornea. The housing includes an interior passageway with an opening at the contact side. The dissection system includes a blade assembly disposed in the interior passageway. The blade assembly includes a first blade and a second blade. The first blade includes a first cutting edge and the second blade includes a second cutting edge. The first blade and the second blade are movable relative to the housing such that the first cutting edge and the second cutting edge extend through the opening of the housing and out of the interior passageway. The first cutting edge produces a first cut in the cornea. The second cutting edge produces a second cut in the cornea. The first cut and the second cut define a volume of tissue for removal from the cornea.

Abstract: A device for separating tissue in an eye includes a body configured to be positioned on the eye surface. The body receives air from one or more air supplies. The body includes one or more air supply channels. The separation device includes a plurality of needles extending from the body. Each needle includes a proximal opening, a distal opening, and a passageway extending between the proximal and distal openings. The proximal openings of the needles are coupled to the one or more air supply channels. The distal openings of the needles are spaced from the body to be positioned in eye tissue. The one or more air supply channels direct the air from the one or more air supplies into the proximal openings, through the passageways, and out the distal openings of the needles and into the eye tissue. The air applies a pressure to separate the eye tissue.