Abstract: Synthesis of semi-synthetic monobactamic or .beta.-Lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a thermodynamically controlled strategy in monophase water/cosolvent organic apolar systems, wherein the concentration of the cosolvent varies between 30% and 90%, the temperature between -10.degree. C. and 50.degree. C., the pH between 4.5 and 8.5, with concentrations of the antibiotic nucleus between 0.5 an 875 mM and acyl donor between 0.2 mM and 1M, with a relationship antibiotic ring/activated or free acyl donor, using a buffer between 0 and 1M. Application to the pharmaceutical industry.
Type:
Grant
Filed:
August 2, 1991
Date of Patent:
December 7, 1993
Assignee:
Cosejo Superior de Invetigaciones Certificas
Inventors:
Jose M. Guisan Seijas, Roberto Fernandez Lafuente, Gregorio Alvaro Campos, Rosa M. Blanco Martin, Cristina Molina Rosell