Abstract: Cotton-containing textiles, such as “trash” feedstock in terms of end-of-life-cotton textiles, may be used to produce sugar without the same kinds of harsh pretreatments used for other biomasses, such as corn, grass sources, or wood. Disclosed is a process for production of sugar from a cotton-containing textile waste fabric comprising optionally mechanically pretreating the cotton-containing textile, pretreating the cotton-containing textile with an acid pretreatment to form a slurry, cooling the slurry, adding at least one base to the slurry, adding at least one additional acid to the slurry to form a buffer in situ, adding a hydrolysis enzyme, and optionally filtering the slurry.

Abstract: This application provides a method for transformation of cells of plant tissues and the regeneration of these cells and tissues into mature transgenic plants. The apical meristematic region of a plant seedling are isolated, then treated to slow the metabolic activity of the isolated tissue. The tissue is then exposed to a transforming agent such as a DNA plasmid or an Agrobacterium carrying a plasmid vector which contains a DNA encoding a gene of interest. After an appropriate incubation period, the treatment is reversed, allowing the metabolic activity of the apical meristematic tissues to return to normal. The method provides a larger population of receptive cells for exposure to the transforming agents, thereby providing higher probabilities of isolating transformed tissues which can be regenerated into transformed plants.