Abstract: The present invention relates to a two-stage sintering method for preparing a porous biphasic calcium phosphate ceramic from calcium-containing biological waste, wherein hydroxyapatite prepared from calcium-containing waste is mixed with a foaming agent to prepare a bone graft material having medicinal use through two-stage sintering.

Abstract: Provided is a method of treating or preventing adenovirus and enterovirus infection by administering an antiviral composition that contains an Echinacea purpurea extract, a Salvia miltiorrhiza extract, or combinations thereof. The antiviral composition blocks adenoviruses and enterovirus infection through virucidal activity against adenovirus and inhibition of adenovirus and enterovirus attachment to and penetration into cells. Also provided is a method of preparing the antiviral composition.

Abstract: A method and system for detecting pesticide residue in agricultural products. The system includes a chromatographic and mass-spectrometric system and a computer. The system inspects first and second sample solutions prepared from two identical extracts of an agricultural product. The first sample solution includes no additives while the second sample is added with a pesticide standard which is intended to be detected by the system for comparison. The computer compares the mass chromatograms of the first and second sample solutions, and calculate an increased integral area by which the peak of the second sample solution in the second mass chromatogram exceeds the peak of the first sample solution in the first mass chromatogram at the same retention time. As such, the concentration of pesticide residue in the sample can be determined by a ratio between the increased integral area and the concentration of the pesticide standard.

Abstract: The present invention provides a quick extraction kit adapted to a procedure of detecting pesticide residues in agricultural products and a method of obtaining a primary test liquid from an agricultural sample by the quick extraction kit. The quick extraction kit comprises a pipe, a first powder mixture layer and a second powder mixture layer. The method of taking primary test liquid is performed as follows. First, obtaining fragments of the agricultural sample. Second, adding an extraction solvent into the fragments of the agricultural sample to obtain a sample solution. Third, adding the sample solution into the pipe. Finally, driving the sample solution to export from the pipe to become the primary test liquid. The quick extraction kit and the method solve the problem of being unable to quickly obtain the result of detecting pesticide residues.

Abstract: Provided herein are a hybridoma cell line producing monoclonal antibody against foot-and-mouth disease virus (FMDV), the monoclonal antibody therefrom, reagent and kit for ELISA, and immunoassay method. The hybridoma cell line CmA40 as deposited under American Type Culture Collection patent deposit number PTA-11304 is produced by cell fusion of a parental cell and a myeloma cell line. The parental cell is a splenocyte isolated from the spleen of a mouse immunized by an antigen derived from a 3ABC non-structural protein (NSP) of FMDV. The antigen used here is expressed by a prokaryotic cell. The monoclonal antibody produced by the hybridoma cell line CmA40 as deposited under American Type Culture Collection patent deposit number PTA-11304 can specifically recognize a 3ABC polypeptide and does not cross-react with an antiserum of swine vesicular disease virus.

Abstract: The present invention relates to a heat- and ethylene-inducible plant specific promoter, and its relevant recombinant plasmids and transgenic plants.

Abstract: A flower tissue-specific promoter, and uses thereof, is the promoter for Phalaenopsis 1-aminocyclopropane-1-carboxylic acid synthase, ACC synthase gene PtACS2, and has a sequence as shown in SEQ ID No: 2. The invention further provides a gene expression cassette, which is composed of a promoter having a DNA sequence as SEQ ID No: 2, and a polynucleotide with an open reading frame linked to the 3? end of said promoter, wherein said promoter can activate transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides furthermore a gene expression vector, which is composed of a promoter having a DNA sequence as SEQ ID No: 2. The invention provides further a method for producing a transgenic plant or parts of organ, tissue or cell of the transgenic plant that contain a gene expression cassette described above.

Abstract: A promoter capable of activating expression specifically in both of the actively dividing young tissues and separate-related aging tissues in plants, characterized in that the promoter is a promoter of Oncidium ethylene receptor gene OgERS1, and has a sequence as shown in SEQ ID No: 3. A gene expression cassette is composed of a promoter having a DNA sequence as shown in SEQ ID No: 3, and a polynucleotide that is linked to the 3? terminal of the promoter and has an open reading frame, wherein the promoter can activate the transcription of the polynucleotide in a organism having the gene expression cassette therein. A gene expression vector is composed of a promoter having a DNA sequence as shown in SEQ ID No: 3. A plant or parts of organ, tissue or cell of the plant has been transformed to contain the gene expression cassette described above.

Abstract: The present invention relates to a heat- and ethylene-inducible plant specific promoter, and its relevant recombinant plasmids and transgenic plants.

Abstract: An isolated Bacillus amyloliquefaciens Ba-BPD1 having an Accession No. of DSM 21836 is provided. This novel strain has unique 16S ribosomal RNA sequenced as SEQ ID NO:1 and produces amylase, protease, cellulase and lipase, fibrinolytic enzyme to show their biodegradation capacities. Further, B. amyloliquefaciens Ba-BPD1 produces the antibiotic substances, such as iturin, fengycin and surfactin, and has antimicrobial capacity for inhibiting the fungal or bacterial growth. In conclusion, the novel strain of Bacillus amyloliquefaciens Ba-BPD1 and its products can be applied in agriculture, wastewater treatment, food industry and chemical industry.

Abstract: The present invention provides a rapid RT-PCR detection method and a diagnostic kit for differentiating field isolates of classical swine fever virus (CSFV) from lapinized CSF vaccine viruses in samples. In order to detecting different genotypes of CSF virus, this invention uses a pair (or pairs) of CSF virus specific primers designed from the conserved sequences within the 3?-untranslated region of CSFV which contains an insertion of 12˜13 nucleotides (poly T) in the region of the lapinized CSF vaccine virus, in comparison with the corresponding region of field isolates of CSFV. By the RT-PCR or the RT-PCR followed by a nest-PCR, field isolates of CSFV and lapinized CSF vaccine viruses in samples could be detected directly and quickly and/or differentiated in electrophoresis without further enzymatic digestion or DNA sequencing.

Abstract: The invention is to provide a device for catching phototaxis flying insects, which is utilized by light irradiating to attract the phototaxis flying insects to gather into the device for facilitating catching the phototaxis flying insects, comprises a light reflecting member having a plurality of reflecting portions for reflecting multi-directional diffused light for attracting the phototaxis flying insects; and a catching member for allowing the phototaxis flying insects to be caught and collected.

Abstract: An optical waveguide SPR sensor is adapted for differential measurement. The optical waveguide SPR sensor includes a base, a bottom layer, and at least one set of optical waveguide layers. The set of the optical waveguide layers includes a measuring optical waveguide channel and a reference optical waveguide channel. The measuring optical waveguide channel includes an SPR sensing film layer. The measuring optical waveguide channel and the reference optical waveguide channel are independently configured and substantially parallel one to another. The bottom layer has a refractive index higher than a refractive index of the optical waveguide layer.

Abstract: A simultaneous detection, differentiation and typing system of Newcastle disease and avian influenza viruses is provided. The present invention provides a system, including an oligonucleotide microarray, avian virus specific probes is disposed on the oligonucleotide microarray and the avian viruses include Newcastle disease and avian influenza viruses, and avian virus nucleic acid products, hybridized with the avian virus specific probes on the oligonucleotide microarray. The present invention describes a fast, simultaneous and inexpensive approach to the detection of Newcastle disease virus (NDV) and avian influenza virus (AIV) and possesses good sensitivity and specificity among divergent viruses. The hybridization results on microarrays were clearly identified with the naked eyes, with no further imaging equipment needed. The present invention provides potential for rapid surveillance and differential diagnosis of these two important zoonoses in both wild and domestic birds.

Abstract: A flower tissue-specific promoter, and uses thereof, is the promoter for Phalaenopsis 1-aminocyclopropane-1-carboxylic acid synthase, ACC synthase gene PtACS2, and has a sequence as shown in SEQ ID No: 2. The invention further provides a gene expression cassette, which is composed of a promoter having a DNA sequence as SEQ ID No: 2, and a polynucleotide with an open reading frame linked to the 3? end of said promoter, wherein said promoter can activate transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides furthermore a gene expression vector, which is composed of a promoter having a DNA sequence as SEQ ID No: 2. The invention provides further a method for producing a transgenic plant or parts of organ, tissue or cell of the transgenic plant that contain a gene expression cassette described above.

Abstract: The present invention relates to a method of directed differentiation of porcine embryonic stem cells into specific neural lineages. The present invention also relates to a method for identifying neurogenic stimulator using the said porcine embryonic stem cells.

Abstract: A nano-scaled antifouling paint for boat is fabricated. A few hydrophobic nano-particles are added into the paint to obtain a nanostructure and a low surface energy. Thus, sea creatures are hard to adhere on the boat or are easily removed. Moreover, owing to a low wettability of the paint, a water drag is reduced when the boat sails, and so fuel is saved.

Abstract: The present invention is related to an oral pertussis vaccine and a method for producing pertussis vaccine, wherein the oral pertussis vaccine comprises amino acid sequence of 1094 to 1279 (SEQ ID NO.1) of filamentous hemagglutinin. The method for producing pertussis vaccine comprises following steps: constructing a vector comprising amino acid sequence of 1094 to 1279 (SEQ ID NO. 1) of filamentous hemagglutinin; transforming the vector into Agrobacterium successfully; co-culturing vector containing Agrobacterium with plant; and obtaining successfully a transformed plant as the vaccine. The present invention is also related to a polypeptide containing SEQ ID NO.1.

Abstract: An insect larva aerosol infection method for producing recombinant proteins and baculovirus bio-insecticides is disclosed. A liquid spray of budded form baculoviruses are employed to infect insect larvae in order to reduce the manpower for manual injections and the energy wasted in feeding infection technologies. The invention may be applied to the production of recombinant proteins and baculoviruses as the production platform for bio-insecticides.

Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.