Abstract: The present disclosure provides a hydrophilic poly(meth)acrylate copolymer film for biological test strip, a preparation method thereof and a biological test strip containing the hydrophilic poly(meth)acrylate copolymer film. The hydrophilic poly(meth)acrylate copolymer film includes a substrate and a poly(meth)acrylate copolymer formed on at least one surface of the substrate. The poly(meth)acrylate copolymer is derived from a formulation including an acrylate-functioned oligomer and a (meth)acrylic acid or (meth)acrylate monomer; and an initiator.