Abstract: Systems and methods for separating charged molecules on the basis of their differential electrostatic binding to a charged stationary phase comprise controlling the creation of simultaneous independent or dependent pH and additive gradients.
Type:
Grant
Filed:
August 8, 2008
Date of Patent:
October 30, 2012
Assignee:
Cryobiophysica, Inc.
Inventors:
Latchezar Ivanov Tsonev, Allen Gene Hirsh
Abstract: The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.
Abstract: Systems and methods for separating charged molecules on the basis of their differential electrostatic binding to a charged stationary phase comprise controlling the creation of simultaneous independent or dependent pH and additive gradients.
Abstract: The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.
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