Abstract: The disclosure relates to a method of cryopreserving biological material (such as spermatozoa from aquatic species) in a plurality of dimensionally stable containers, each having two parallel walls defining between them a storage space having a uniform thickness. The method comprises the steps of: at least partially filling the storage space with the biological material via an opening; closing the opening; and freezing the biological material enclosed in the containers to a cryogenic temperature. According to the method, the containers are racked in substantially parallel planes and the freezing step includes bringing the racked containers to a first reservoir (365) and fully immersing the containers in a first freezing agent held therein at a first temperature, and circulating the first freezing agent between the racked containers (e.g. using a rotor or pump).

Abstract: A method for cryopreservation of biological material where a sample of a biological material is inserted into a packaging having two substantially parallel walls spaced from 2.5 mm apart or less, the walls connected to each other along a substantially part of their periphery and including a plurality of internal ridges for directing the flow of material through the packaging. After the material is evenly distributed in the package, the material is frozen for cryopreservation of biological material.