Patents Assigned to Crystal Bioscience Inc.
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Patent number: 11602136Abstract: A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.Type: GrantFiled: January 6, 2020Date of Patent: March 14, 2023Assignee: CRYSTAL BIOSCIENCE INC.Inventor: William Don Harriman
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Patent number: 11542319Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.Type: GrantFiled: May 15, 2020Date of Patent: January 3, 2023Assignee: CRYSTAL BIOSCIENCE INC.Inventors: Philip A. Leighton, William Don Harriman, Robert Etches
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Patent number: 11337409Abstract: This disclosure provides, among other things, strategies for minimizing antibody diversification in a transgenic animal that uses gene conversion for antibody diversification. In some embodiments, the animal may comprise a genome comprising an endogenous immunoglobulin light chain locus comprising: (a) a functional immunoglobulin light chain gene comprising a nucleic acid encoding a light chain variable region; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin light chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding a light chain variable region, wherein the pseudogenes are upstream or downstream of the functional immunoglobulin light chain gene and encode the same amino acid sequence as the light chain variable region of the functional immunoglobulin light chain gene of (a). In other embodiments, the locus may have a tandem array of coding sequences for the light chain.Type: GrantFiled: June 5, 2019Date of Patent: May 24, 2022Assignee: CRYSTAL BIOSCIENCE INC.Inventors: Philip A. Leighton, William Don Harriman, Robert Etches
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Patent number: 11033009Abstract: This disclosure provides, among other things, a transgenic animal and a method of using the same to make antibodies that have a common light chain. In certain embodiments, the transgenic animal may comprising a genome comprising a common light chain transgene, wherein the common light chain transgene comprises a non-immunoglobulin light-chain promoter and a common light-chain coding sequence. In certain embodiments, the common light chain is constitutively expressed.Type: GrantFiled: August 24, 2016Date of Patent: June 15, 2021Assignee: CRYSTAL BIOSCIENCE INC.Inventors: William Don Harriman, Robert Etches, Philip A. Leighton
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Patent number: 10687519Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: GrantFiled: May 31, 2019Date of Patent: June 23, 2020Assignee: CRYSTAL BIOSCIENCE INC.Inventors: William Don Harriman, Robert Etches, Philip A. Leighton
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Patent number: 10689433Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.Type: GrantFiled: October 2, 2017Date of Patent: June 23, 2020Assignee: CRYSTAL BIOSCIENCE INC.Inventors: Philip A. Leighton, William Don Harriman, Robert Etches
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Patent number: 10555508Abstract: A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.Type: GrantFiled: April 30, 2018Date of Patent: February 11, 2020Assignee: CRYSTAL BIOSCIENCE INC.Inventor: William Don Harriman
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Patent number: 10362770Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: GrantFiled: December 4, 2018Date of Patent: July 30, 2019Assignee: Crystal Bioscience Inc.Inventors: William Don Harriman, Robert Etches, Philip A. Leighton
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Patent number: 10172334Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: GrantFiled: June 1, 2018Date of Patent: January 8, 2019Assignee: Crystal Bioscience Inc.Inventors: William Don Harriman, Robert Etches, Philip A. Leighton
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Patent number: 10010058Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: GrantFiled: December 13, 2016Date of Patent: July 3, 2018Assignee: Crystal Bioscience Inc.Inventors: William Don Harriman, Robert Etches, Philip A. Leighton
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Patent number: 9982062Abstract: A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.Type: GrantFiled: June 8, 2016Date of Patent: May 29, 2018Assignee: CRYSTAL BIOSCIENCE INC.Inventor: William Don Harriman
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Patent number: 9809642Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.Type: GrantFiled: May 27, 2016Date of Patent: November 7, 2017Assignee: Crystal Bioscience Inc.Inventors: Philip A. Leighton, William Don Harriman, Robert Etches
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Patent number: 9644178Abstract: A sustained culture of isolated avian gonocytes is provided, as well as a method of making and using the same. A chimeric avian containing an isolated gonocyte and a transgenic avian produced using the chimeric avian are also provided. The cell and method may be employed to make, among other things, transgenic avian that produce a heterologous protein, e.g., a therapeutic protein.Type: GrantFiled: September 16, 2014Date of Patent: May 9, 2017Assignee: CRYSTAL BIOSCIENCE, INC.Inventors: Marie-Cecile van de Lavoir, Robert Etches
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Patent number: 9549538Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: GrantFiled: June 21, 2016Date of Patent: January 24, 2017Assignee: CRYSTAL BIOSCIENCE, INC.Inventors: William Don Harriman, Robert Etches, Philip A. Leighton
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Patent number: 9404125Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: GrantFiled: October 18, 2013Date of Patent: August 2, 2016Assignee: CRYSTAL BIOSCIENCE, INC.Inventors: William Don Harriman, Robert Etches, Phil Leighton
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Patent number: 9394372Abstract: A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.Type: GrantFiled: October 16, 2012Date of Patent: July 19, 2016Assignee: CRYSTAL BIOSCIENCE INC.Inventor: William Don Harriman
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Patent number: 9380769Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.Type: GrantFiled: May 23, 2012Date of Patent: July 5, 2016Assignee: Crystal Bioscience Inc.Inventors: Phil Leighton, William Don Harriman, Robert Etches
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Patent number: 8865462Abstract: A sustained culture of isolated avian gonocytes is provided, as well as a method of making and using the same. A chimeric avian containing an isolated gonocyte and a transgenic avian produced using the chimeric avian are also provided. The cell and method may be employed to make, among other things, transgenic avian that produce a heterologous protein, e.g., a therapeutic protein.Type: GrantFiled: January 7, 2011Date of Patent: October 21, 2014Assignee: Crystal Bioscience Inc.Inventors: Marie-Cecile van de Lavoir, Robert Etches
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Publication number: 20140082759Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immuno -well as globulin gene is provided, as we cells and targeting vectors for making the same.Type: ApplicationFiled: May 23, 2012Publication date: March 20, 2014Applicant: Crystal Bioscience Inc.Inventors: Phil Leighton, William Don Harriman, Robert Etches
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Publication number: 20140068796Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.Type: ApplicationFiled: October 18, 2013Publication date: March 6, 2014Applicant: CRYSTAL BIOSCIENCE, INC.Inventors: William Don Harriman, Robert Etches, Phil Leighton