Abstract: A system and method for solid phase chemical synthesis reduces the overall amount of solvent required for the process. In, for example, solid phase peptide synthesis, during deprotection of the bound peptide, a deprotecting agent, such as piperidine in dimethylformamide (DMF) is introduced to the reaction vessel. After deprotection is complete, pure DMF, or other similar wash solvent, is used to wash away the residual piperidine from the reaction vessel. The used wash solvent can be removed from the reaction vessel, warmed to evaporate off the piperidine, and the purified used wash solvent can be cooled and re-introduced into the reaction vessel for a subsequent washing. Thus, instead of using six volumes of DMF for washing six times, for example, a single volume of DMF can be used, purified and reused, thus substantially reducing the volume of DMF needed for washing the deprotecting agent from the reaction vessel.

Abstract: A peptide synthesis instrument can be used for small scale peptide synthesis. The instrument can include several unique features, including a compression style reaction vessel permitting quick setup of the reaction vessel, a double reaction vessel system permitting efficient mixing without loss of solvent or solvent-to-resin contact, gravity-fed heated reservoirs establishing a fixed volume for delivery to the reaction vessel, fume-free solvent addition permitting solvent addition to fixed bottles, and an improved amino acid manifold assembly which reduces the number of components and increases the ease of use of the instrument. Each of these features improve upon the current state of the art in solid phase automated peptide synthesizers.

Abstract: A peptide synthesis instrument can be used for small scale peptide synthesis. The instrument can include several unique features, including a compression style reaction vessel permitting quick setup of the reaction vessel, a double reaction vessel system permitting efficient mixing without loss of solvent or solvent-to-resin contact, gravity-fed heated reservoirs establishing a fixed volume for delivery to the reaction vessel, fume-free solvent addition permitting solvent addition to fixed bottles, and an improved amino acid manifold assembly which reduces the number of components and increases the ease of use of the instrument. Each of these features improve upon the current state of the art in solid phase automated peptide synthesizers.

Abstract: A peptide synthesis instrument can be used for small scale peptide synthesis. The instrument can include several unique features, including a compression style reaction vessel permitting quick setup of the reaction vessel, a double reaction vessel system permitting efficient mixing without loss of solvent or solvent-to-resin contact, gravity-fed heated reservoirs establishing a fixed volume for delivery to the reaction vessel, fume-free solvent addition permitting solvent addition to fixed bottles, and an improved amino acid manifold assembly which reduces the number of components and increases the ease of use of the instrument. Each of these features improve upon the current state of the art in solid phase automated peptide synthesizers.