Abstract: The present invention relates to a method for extracting nucleic acids from a biological sample, and the extraction method presents a novel method for effectively extracting nucleic acids. When nucleic acids are extracted from biological samples in the related art, various impurities present in the biological samples are not properly removed, such that the purification rate is low, but the present invention provides a method for extracting nucleic acids from a biological sample of which the bacteria, virus and nucleic acid recovery rates are enhanced, by adding a surfactant and a sodium sulfate (Na2SO4) solution in a biological sample disruption step and a purification step, thereby enabling pathogens to be detected more sensitively and accurately.