Abstract: A method, apparatus and computer program product to provide machine learning-assisted detection of objects of interest in live cell-based assays, where the objects of interest are larger than cells used in the assay. The technique herein comprises receiving image data that has been captured from a set of imaging devices, such as immune monitoring analyzer machines. Representative image data is an enzyme-linked immune absorbent spot (ELISPOT) assay captured from an ELISPOT analyzer. For each set of image data, the image data is then processed using, for example, one of: (a) a first pre-trained model; and (b) a set of one or more detection algorithms, to generate training data comprising a set of labels for the image data. A model, e.g., a deep neural network (DNN), is then trained using the set of labels. Following training, the model is provided for detection of the objects of interest.

Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.

Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.