Abstract: The present invention relates to a method for determining an estimate of a concentration of particles E(C), wherein a sample of predetermined volume is divided into a number (N) of compartments, the (N) compartments comprise or consist of different sample volumes (v1) and/or different dilution factors (di) of the sample, at least part of the particles that are present in any of the (N) compartments provide a measurable signal and the estimated concentration of particles E(C) is a function of measured signals, as well as an apparatus for use in the inventive method, uses of the inventive method, a sample holder and a kit for use in the inventive method.