Abstract: The present invention relates to a calibration device and method for use in calibrating a thermal cycler having a reaction zone (140), an excitation light source (120) and an optical detector (110), the device comprising one or more ambient condition sensors (210?, 230?), each adapted to sense an ambient condition at a respective position within said reaction zone, one or more emission light generators (220?) adapted to be in optical communication with the optical detector (110), and control circuitry coupled to the one or more ambient condition sensors and to the one or more emission light generators, wherein the control circuitry is configured to alter the emission light generated by the one or more emission light generators based on the ambient condition sensed by the one or more ambient condition sensors.

Abstract: The present invention relates to a calibration device and method for use in calibrating a thermal cycler having a reaction zone (140), an excitation light source (120) and an optical detector (110), the device comprising one or more ambient condition sensors (210?, 230?), each adapted to sense an ambient condition at a respective position within said reaction zone, one or more emission light generators (220?) adapted to be in optical communication with the optical detector (110), and control circuitry coupled to the one or more ambient condition sensors and to the one or more emission light generators, wherein the control circuitry is configured to alter the emission light generated by the one or more emission light generators based on the ambient condition sensed by the one or more ambient condition sensors.