Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using recombinant host cells. More particularly, the present invention pertains to recombinant host cells comprising (e.g., expressing) a polypeptide having aryl sulfotransferase activity, wherein said recombinant host cells have been modified to have an increased uptake of sulfate compared to identical host cells that does not carry said modification. Further provided are processes for the production of aryl sulfates, such as zosteric acid, employing such recombinant host cells.
Type:
Grant
Filed:
January 27, 2021
Date of Patent:
January 17, 2023
Assignee:
CysBio ApS
Inventors:
Christian Bille Jendresen, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.
Type:
Grant
Filed:
October 6, 2020
Date of Patent:
August 9, 2022
Assignee:
CysBio ApS
Inventors:
Hemanshu Mundhada, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using polypeptides or recombinant cells comprising said polypeptides. More particularly, the present invention pertains to polypeptides having aryl sulfotransferase activity, recombinant host cells expressing same and processes for the production of aryl sulfates employing these polypeptides or recombinant host cells.
Type:
Grant
Filed:
July 30, 2020
Date of Patent:
July 19, 2022
Assignee:
CysBio ApS
Inventors:
Christian Bille Jendresen, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of hydroxycinnamic acids using polypeptides having tyrosine ammonia lyase activity. More particularly, the present invention pertains to polypeptides having tyrosine ammonia lyase activity and high substrate specificity towards tyrosine, which makes them particularly suitable in the production of p-coumaric acid and other hydroxycinnamic acids. The present invention thus provides processes for the production of p-coumaric acid and other hydroxycinnamic acids employing these polypeptides as well as recombinant host cells expressing same.
Type:
Grant
Filed:
July 24, 2020
Date of Patent:
April 19, 2022
Assignee:
CYSBIO APS
Inventors:
Christian Bille Jendresen, Solvej Siedler, Steen Gustav Stahlhut, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using recombinant host cells. More particularly, the present invention pertains to recombinant host cells comprising (e.g., expressing) a polypeptide having aryl sulfotransferase activity, wherein said recombinant host cells have been modified to have an increased uptake of sulfate compared to identical host cells that does not carry said modification. Further provided are processes for the production of aryl sulfates, such as zosteric acid, employing such recombinant host cells.
Type:
Grant
Filed:
February 24, 2017
Date of Patent:
March 23, 2021
Assignee:
CysBio ApS
Inventors:
Christian Bille Jendresen, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.
Type:
Grant
Filed:
January 27, 2016
Date of Patent:
October 6, 2020
Assignee:
CysBio Aps
Inventors:
Hemanshu Mundhada, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of hydroxycinnamic acids using polypeptides having tyrosine ammonia lyase activity. More particularly, the present invention pertains to polypeptides having tyrosine ammonia lyase activity and high substrate specificity towards tyrosine, which makes them particularly suitable in the production of p-coumaric acid and other hydroxycinnamic acids. The present invention thus provides processes for the production of p-coumaric acid and other hydroxycinnamic acids employing these polypeptides as well as recombinant host cells expressing same.
Type:
Grant
Filed:
July 14, 2015
Date of Patent:
August 25, 2020
Assignee:
CYSBIO APS
Inventors:
Christian Bille Jendresen, Solvej Siedler, Steen Gustav Stahlhut, Alex Toftgaard Nielsen
Abstract: The present invention relates to the production of hydrolyzates from a lignocellulose-containing material, and to fermentation of the hydrolyzates. More specifically, the present invention relates to the detoxification of phenolic inhibitors and toxins formed during the processing of lignocellulose-containing material by enzymatically sulfating the phenolic inhibitors and toxins using aryl sulfotranseferases.
Type:
Grant
Filed:
August 21, 2015
Date of Patent:
March 17, 2020
Assignee:
CysBio ApS
Inventors:
Christian Bille Jendresen, Alex Toftgaard Nielsen
Abstract: The present invention generally relates to the microbiological industry, and specifically to the production of L-serine using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.
Type:
Grant
Filed:
January 27, 2016
Date of Patent:
December 24, 2019
Assignee:
CYSBIO APS
Inventors:
Hemanshu Mundhada, Alex Toftgaard Nielsen