Patents Assigned to CYTIVA BIOPROCESS R&D AB
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Patent number: 11141681Abstract: The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.Type: GrantFiled: March 14, 2017Date of Patent: October 12, 2021Assignee: Cytiva BioProcess R&D ABInventor: Klaus Gebauer
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Patent number: 11136359Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.Type: GrantFiled: February 20, 2018Date of Patent: October 5, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav Rodrigo, Mats Ander, Tomas Bjorkman, Goran Bauren
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Patent number: 11136357Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more mutated binding domains of Peptostreptococcus Protein L.Type: GrantFiled: January 18, 2019Date of Patent: October 5, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav Rodrigo, Mats Ander, Tomas Bjorkman
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Patent number: 11124539Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.Type: GrantFiled: November 14, 2017Date of Patent: September 21, 2021Assignee: Cytiva BioProcess R&D ABInventors: Johan William Ohman, Mats Ander, Therese Graner, Anneli Jorsback, Jinyu Zou
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Patent number: 11053283Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.Type: GrantFiled: April 17, 2019Date of Patent: July 6, 2021Assignee: Cytiva BioProcess R&D ABInventors: Jean-Luc Maloisel, Ola Lind, Bjorn Noren, Ronnie Palmgren
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Patent number: 11027250Abstract: A system and method for producing fine droplets of polysaccharide from a premixed water-in-oil emulsion uses a packed bed (5) comprising hydrophilic beads (7).Type: GrantFiled: September 4, 2017Date of Patent: June 8, 2021Assignee: Cytiva BioProcess R&D ABInventors: Jimmy Hedin Dahlstrom, Jonas Bengtsson, Per Erik Emilsson, Maranda Fordham, Eva Holmgren, Ronnie Palmgren, Fernando Polanco
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Patent number: 11014069Abstract: The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.Type: GrantFiled: October 28, 2016Date of Patent: May 25, 2021Assignee: Cytiva BioProcess R&D ABInventors: Nils Norrman, Mattias Algotsson, Stefan D. Eriksson
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Patent number: 10995113Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: June 5, 2020Date of Patent: May 4, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Patent number: 10954309Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.Type: GrantFiled: November 11, 2015Date of Patent: March 23, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav Rodrigo, Tomas Bjorkman, Mats Ander
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Patent number: 10940403Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b) connected in a rigid housing (21; 61), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.Type: GrantFiled: April 16, 2018Date of Patent: March 9, 2021Assignee: Cytiva BioProcess R&D ABInventors: Klaus Gebauer, Manoj Kumar Ramakrishna, Daniel Salomonsson, Per Lofving
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Patent number: 10933350Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.Type: GrantFiled: October 8, 2018Date of Patent: March 2, 2021Assignee: Cytiva BioProcess R&D ABInventor: Klaus Gebauer
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Patent number: 10928365Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.Type: GrantFiled: July 1, 2019Date of Patent: February 23, 2021Assignee: Cytiva BioProcess R&D ABInventor: Klaus Gebauer
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Patent number: 10918971Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.Type: GrantFiled: December 2, 2016Date of Patent: February 16, 2021Assignee: Cytiva BioProcess R&D ABInventor: Sophia Hober
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Patent number: 10894241Abstract: A method for forming polymer microparticles is disclosed. The method comprises spraying a liquid comprising a thermally-gelling polymer from a first orifice of a nozzle into air to form gelled microparticles, and collecting the gelled microparticles with a collecting device. The nozzle is positioned to have a central axis of the first orifice at a tilt angle in a range from about 10 degrees to about 170 degrees relative to a direction of gravity. The collecting device comprises at least two collecting units at different distances from the first orifice of the nozzle in a horizontal direction perpendicular to the direction of gravity. Each of the collecting unit is loaded with a liquid collecting medium. A system for forming polymer microparticles is also disclosed.Type: GrantFiled: June 22, 2017Date of Patent: January 19, 2021Assignee: Cytiva BioProcess R&D ABInventors: Lin Chen, Jie Gao, Qiongqiong Huang
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Patent number: 10889615Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52, wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO: 4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.Type: GrantFiled: May 10, 2017Date of Patent: January 12, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander
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Patent number: 10875007Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.Type: GrantFiled: June 17, 2019Date of Patent: December 29, 2020Assignee: CYTIVA BIOPROCESS R&D ABInventors: Martin Hall, Sture Larsson, Andreas Muranyi, Gustav Rodrigo, Jinyu Zou, Per-Mikael Aberg
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Patent number: 10864512Abstract: The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles (1) having a core region (2) and a shell region (3), wherein: a) said shell region is accessible to a target biomacromolecule; b) said core region is less accessible to the target biomacromolecule than the shell region; and c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.Type: GrantFiled: March 30, 2017Date of Patent: December 15, 2020Assignee: Cytiva BioProcess R&D ABInventors: Jean-Luc Maloisel, Tobias Söderman, Ola Lind, Ida Eklind
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Patent number: 10830740Abstract: A method in a separation system including parallel fluid paths each having a separation module, includes providing a sensor of the same type in at least each of the parallel fluid paths except one: measuring a characteristic fluid property with at least one of the sensors in the parallel fluid paths; possibly measuring the same characteristic fluid property with a system sensor positioned in the outlet of the separation system; and comparing measured characteristic fluid properties to evaluate and/or qualify the performance of the separation system.Type: GrantFiled: March 30, 2011Date of Patent: November 10, 2020Assignee: CYTIVA BIOPROCESS R&D ABInventor: Klaus Gebauer