Abstract: Disclosed is a method of detecting a nucleic acid sequence of interest in a sample, the method comprising contacting the sample with first and second probes; the first probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest; the second probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest but complementary to that portion of the first probe which is non-complementary to the sequence of interest, such that the first and second probes are capable of hybridising to the sequence of interest in an adjacent or substantially adjacent manner, so as to allow complementary portions of the first and second probes to hybridize to each other; causing extension of the first probe with a nucleic acid polymerase, using the second probe as a template; and detecting directly or indirectly the extension of
Type:
Grant
Filed:
July 19, 1999
Date of Patent:
May 21, 2002
Assignee:
Cytocell Limited
Inventors:
Anthony Weston, Rene Assenberg, Peter Marsh, Graham A Mock, Trevor D Ray, Susan D Wharam, Donald L. N. Cardy
Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of:
(a) adding first and second nucleic acid probes to a sample comprising the sequence of interest, so as to form a complex comprising three strands of nucleic acid, wherein the first probe comprises the full length sequence of a first strand of a double stranded promoter, the target sequence comprises an end part of a second strand of the double stranded promoter which is complementary to a part of the first strand, and the second probe comprises the rest of the second strand of the double stranded promoter which is complementary to a part of the first strand, such that a functional promoter is formed when the first probe is hybridized to both the target sequence and to the second probe;
(b) adding a polymerase which recognizes the promoter, so as to cause the de novo synthesis of nucleic acid from the promoter present in the complex; and
(c) detecting directly or indirectly the
Type:
Grant
Filed:
April 16, 1999
Date of Patent:
September 11, 2001
Assignee:
Cytocell Limited
Inventors:
Anthony Weston, Rene Assenberg, Peter Marsh, Graham A Mock, Trevor D. Ray, Susan D Wharam, Donald L. N. Cardy
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