Abstract: Peripheral blood lymphocytes displayed a plurality of sizes and colors when exposed first to a fixative solution of C.I. basic blue 141, then to an aqueous alkaline solution of the same dye and subsequently treated with a neutral HEPES buffer solution containing small but effective amounts of various metal compounds. As confirmed with purified lymphocyte subpopulations obtained with a cell sorter, T helper cells (CD4) were small and their nuclei and cytoplasm stained deep blue. T cytotoxic/suppressor cells (CDS) were larger than T helper cells, their nuclei stained pale green or blue green and their cytoplasm contained a cluster of magenta colored granules. Used in the process described, basic blue 141 provides means of identifying and differentiating CD4 and CD8 cells under the ordinary light microscope without using monoclonal antibodies or fluorescence.

Abstract: This invention is directed to the cytology of blood, bone marrow and lymph node cells and to the method of differentiating, identifying and enumerating monocytes among a plurality of cells of hematopoietic origin. More specifically, the invention is directed to stained cells and to the use of a cationic sulfur-containing azo dye capable of staining monocytes a bright red-violet color. The dye is a water soluble quaternary azo dye identified in the Colour Index as Basic Blue 54 (C.I. 11052). The stained monocytes obtained by the process of this invention have color characteristics which permit the identification and differentiation of said cells by use of various instruments including, for example, an image analyzer, microscope, photo microscope, and the like.

Abstract: This invention relates to the cytology of blood, bone marrow, and lymph node cells, and to the composition and method of differentiating, identifying, and enumerating said cells among a plurality of cells of hematopoietic origin. More specifically, the invention is directed to the use of basis cationic diazo dyes capable of staining a plurality of cells of hematopoietic origin to differentiate and enumerate the abnormal from the normal cells. The stained cells obtained by this invention have individual color characteristics which permit the identification and differentiation by use of various optical instruments, including, for example, an image analyzer, microscope, photomicroscope and the like.

Abstract: This invention relates to the cytology of blood, bone marrow, and lumpth node cells, and a method of differentiating, identifying, and enumerating said cells among a plurality of cells of hematopoietic origin. More specifically, the invention is directed to the use of basic cationic diazo dyes capable of staining a plurality of cells of hematopoietic origin to differentiate and enumerate the abnormal from the normal cells. The stained cells obtained by this invention have individual color characteristics which permit the identification and differentiation by use of various optical instruments, including, for example, an image analyzer, microscope, photomicroscope and the like.

Abstract: This invention is directed to the use of specific polymethine dyes for staining fixed biopsy specimens of hematopoietic origin consisting of blood, bone marrow and lymph node cells for purposes of identifying and enumerating the stained cells when viewed under dark field illumination. The polymethine dyes, e.g. Astrazon Red Violet FRR, stain the lymphocyte subpopulations distinct colors capable of being viewed under dark field illumination for purposes of distinguishing one stained cell from another. This method of staining and the compositions thereof represents an advance in the art of lymphocyte subpopulation identification which was achieved heretofore primarily by using monoclonal antibody techniques.