Abstract: A method for isolating urea and removing CO2 from plasma samples, comprising the following steps: a) providing a plasma sample; b) adding an acid so as to partially remove CO2; c) lyophilizing the sample so as to further remove CO2 and obtain a dried sample; and d) redissolving the dried sample and neutralizing to a pH value of 4 to 7 using a buffer solution, wherein optionally a filtration step is carried out before adding the acid.
Abstract: In a process for preparing disinfected preparations from mammalian tissues, mammalian tissue is perfused at one step with an enzyme-free liquid antibiotic composition that includes at least one antibiotic and is contained in at least one perfusion buffer. In a step occurring after such one step, enzymatic antibiotic-free treatment of the tissue is carried out to obtain a single-cell suspension. In another step disinfected preparations are obtained.
Type:
Grant
Filed:
November 12, 2014
Date of Patent:
July 10, 2018
Assignee:
Cytonet GmbH & Co. KG
Inventors:
Krasimira Aleksandrova, Marc Barthold, Lubomir Arseniev, Carsten Griesel, Christoph Priesner
Abstract: Microcapsules including a capsule shell encapsulating a suspension of a therapeutically effective amount of liver cells in physical contact with a liver cell stimulating amount of erythropoietin.
Type:
Grant
Filed:
September 16, 2013
Date of Patent:
December 13, 2016
Assignee:
CYTONET GMBH & CO. KG
Inventors:
Krasimira Aleksandrova, Peter Pediaditakis, Jo Salisbury, Wolfgang Rüdinger
Abstract: In a process for preparing disinfected preparations from mammalian tissues, mammalian tissue is perfused at one step with an enzyme-free liquid antibiotic composition that includes at least one antibiotic and is contained in at least one perfusion buffer. In a step occurring after such one step, enzymatic antibiotic-free treatment of the tissue is carried out to obtain a single-cell suspension. In another step disinfected preparations are obtained.
Type:
Grant
Filed:
July 30, 2011
Date of Patent:
April 7, 2015
Assignee:
Cytonet GmbH & Co. KG
Inventors:
Krasimira Aleksandrova, Marc Barthold, Lubomir Arseniev, Carsten Griesel, Christoph Priesner
Abstract: Microcapsules including a capsule shell encapsulating a suspension of a therapeutically effective amount of liver cells in physical contact with a liver cell stimulating amount of erythropoietin.
Type:
Application
Filed:
September 16, 2013
Publication date:
January 16, 2014
Applicant:
CYTONET GMBH & CO. KG
Inventors:
Krasimira ALEKSANDROVA, Peter PEDIADITAKIS, Jo SALISBURY, Wolfgang RÜDINGER
Abstract: Microcapsules including a capsule shell encapsulating a suspension of a therapeutically effective amount of liver cells in physical contact with a liver cell stimulating amount of erythropoietin.
Type:
Grant
Filed:
April 27, 2010
Date of Patent:
September 17, 2013
Assignee:
Cytonet GmbH & Co. KG
Inventors:
Krasimira Aleksandrova, Peter Pediaditakis, Jo Salisbury, Wolfgang Rüdinger
Abstract: A method for isolating urea and removing CO2 from plasma samples, comprising the following steps: a) providing a plasma sample; b) adding an acid so as to partially remove CO2; c) lyophilizing the sample so as to further remove CO2 and obtain a dried sample; and d) redissolving the dried sample and neutralizing to a pH value of 4 to 7 using a buffer solution, wherein optionally a filtration step is carried out before adding the acid.
Abstract: The invention relates to a process for the preparation of disinfected single-cell preparations from mammalian tissues and to the preparations thus prepared.
Type:
Application
Filed:
July 30, 2011
Publication date:
June 6, 2013
Applicant:
Cytonet GmbH & Co. KG
Inventors:
Krasimra Aleksandrova, Marc Barthold, Lubomir Arseniev, Carsten Griesel, Christoph Priesner
Abstract: The invention relates to a method for storages of human cells in particular, cell media for storage of cells and the use of such cell media for storage of cells, wherein the vitality of the cells is preserved during the storage.
Type:
Application
Filed:
June 30, 2006
Publication date:
August 21, 2008
Applicant:
Cytonet GmbH & Co. KG
Inventors:
Lubomir Arseniev, Krassimira Alexandrova, Marc Barthold, Carsten Griesel, Hans-Gerd Heuft, Sabine Kafert-Kasting, Christoph Priesner