Abstract: The present invention provides a disposable microfluidic assay cartridge (1) which will contain at least one sample inlet well (2) that will feed into a microfluidic sub-unit (3) embedded within the disposable microfluidic assay cartridge (1). The microfluidic sub-unit (3) contains a series of microfluidic channels and micro-valves (4) that direct the sample from the sample inlet well (2) to separate and fluidicly-isolated reaction vessels (5) that contain encoded or non-encoded beads microparticles (6) which have been functionalized with a capture moiety or capture molecules such as antibodies, antigens, or oligomers. Assay reagents (7) including reagents R1, R2, R3, R4, such as labeled antibodies, will be introduced into the separate and fluidicly-isolated reaction vessels (5) via the series of microfluidic channels (8) and micro-valves (4).