Abstract: A chromogenic assay for determination of blood coagulation Factor VIII:Ca, using an indicator of Factor Xa simultaneously as a measure of Factor VIII:Ca concentration and as an inhibitor of Factor Xa. This technique can be applied to measure the concentration of an activating enzyme using the rate of conversion of the indicator molecule of the product of that enzyme as an indirect indicator of enzyme concentration.
Type:
Grant
Filed:
January 9, 1997
Date of Patent:
August 8, 2000
Assignee:
Dade Behring AG
Inventors:
Hendrik Coenraad Hemker, Hendricus Johannes Kessels, Suzette Lucette BeGuin