Abstract: Disclosed herein are a collagen-based matrix for use as a restorative material and a method for the preparation thereof. An atelocollagen dispersion is spread at a predetermined thickness over a plate and freeze-dried to form a porous collagen membrane. An atelocollagen dispersion is separately spread over a plate and pressurized to form a dense collagen membrane. This is overlaid with the porous collagen membrane and immersed in an EDS solution in ethanol to crosslink the two membranes with each other. From the bilayer structure thus constructed, EDS is removed, followed by lyophilization and cutting into an appropriate size.
Type:
Grant
Filed:
April 15, 2009
Date of Patent:
June 10, 2014
Assignee:
Dalim Tissen Inc.
Inventors:
Seong-Ki Lee, Si-Nae Park, Sang-Hee Bae
Abstract: The present invention provides a method for separating an atelocollagen, wherein the process of ultrafiltration utilizing reusable filters and the process of diafiltration are combined, and by way of a single procedure line, atelocollagens can be extracted in a high purity from an animal tissue economically by removing impurities efficiently and conveniently.
Abstract: Disclosed herein are a collagen-based matrix for use as a restorative material and a method for the preparation thereof. An atelocollagen dispersion is spread at a predetermined thickness over a plate and freeze-dried to form a porous collagen membrane. An atelocollagen dispersion is separately spread over a plate and pressurized to form a dense collagen membrane. This is overlaid with the porous collagen membrane and immersed in an EDS solution in ethanol to crosslink the two membranes with each other. From the bilayer structure thus constructed, EDS is removed, followed by lyophilization and cutting into an appropriate size.
Type:
Application
Filed:
April 15, 2009
Publication date:
February 18, 2010
Applicant:
DALIM TISSEN Inc.
Inventors:
Seong-Ki LEE, Si-Nae Park, Sang-Hee Bae