Abstract: Provided is a culture medium composition including turmeric, glycine, or insulin for culturing muscle stem cells for proliferation of muscle stem cells. According to the culture medium composition including turmeric, glycine, or insulin muscle stem cells for culturing muscle stem cells according to an aspect, the proliferation and differentiation abilities of cells do not degrade even when culturing muscle stem cells in vitro, resulting in effects of enabling mass culture of muscle stem cells. Accordingly, the culture medium composition is economical by effectively replacing a culture medium containing expensive bFGF, and since appropriate time of differentiation can be chosen when culturing muscle stem cells, the muscle stem cells can be differentiated when needed, thereby producing an effect of improving the quality of in vitro meat.

Abstract: This application relates to an automated three-dimensional (3D) cell culture system. The system includes a first body including a cell culture space in which a scaffold, on which cells are cultured, is provided and the cells are cultured thereon, and a medium storage space which is partitioned from the cell culture space by a first partition wall, in which a medium is provided and stored, and which surrounds the cell culture space, wherein the first partition wall has a drain hole to communicate the medium storage space with the cell culture space. The system also includes a communication pipe provided at the first partition wall to drain the medium of the cell culture space to the outside by a siphon action. The system further includes a second body including a medium recovery space located under the first body and recovering and externally draining the medium drained through the communication pipe.