Patents Assigned to Danisco Ingredients A/S (Danisco A/S)
  • Method to isolate mutants and to clone the complementing gene
    Patent number: 6599745
    Abstract: The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.
    Type: Grant
    Filed: July 11, 2000
    Date of Patent: July 29, 2003
    Assignee: Danisco Ingredients A/S (Danisco A/S)
    Inventors: Leendert Hendrik De Graaff, Henriëtta Catharina Van Den Broeck, Jacob Visser
  • &bgr;-xylosidase, nucleotide sequence encoding it, and use thereof
    Patent number: 6300112
    Abstract: A nucleotide sequence is provided which encodes a peptide having &bgr;-xylosidase activity and exhibits at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or hybridises under stringent conditions with a nucleotide sequence shown in SEQ ID NO. 1, or a part thereof having at least 15 nucleotides encoding an amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3. Also provided is a peptide having &bgr;-xylosidase activity and exhibiting at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or a part thereof having at least 8 amino acids shown in SEQ ID NO. 1 and described in SEQ ID NO. 3.
    Type: Grant
    Filed: December 22, 1997
    Date of Patent: October 9, 2001
    Assignee: Danisco Ingredients A.S (Danisco A/S)
    Inventors: Leendert Hendrik De Graaff, Noël Nicolaas Maria Elisabeth Van Peij, Henriëtta Catharina Van Den Broeck, Jacob Visser
  • Method to isolate mutants and to clone the complementing gene
    Patent number: 6177261
    Abstract: The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant.
    Type: Grant
    Filed: December 23, 1997
    Date of Patent: January 23, 2001
    Assignee: Danisco Ingredients A/S (Danisco A/S)
    Inventors: Leendert Hendrik De Graaff, Henri{umlaut over (e)}tta Catharina Van Den Broeck, Jacob Visser