Abstract: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
Type:
Grant
Filed:
June 7, 2011
Date of Patent:
January 3, 2017
Assignee:
DANISCO US IN.
Inventors:
Philippe Soucaille, Marguerite A. Cervin, Fernando Valle