Patents Assigned to Danstar Ferment AG
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Publication number: 20260176656Abstract: The present disclosure concerns a method of obtaining a composition having yeast proteins derived from yeast protoplasts. The present disclosure also includes compositions comprising yeast proteins derived from yeast protoplasts, edible products derived from said composition as well as usages thereof.Type: ApplicationFiled: October 26, 2023Publication date: June 25, 2026Applicant: DANSTAR FERMENT AGInventors: Stanislaw PTASZYNSKI, Jan-Martijn HAMELINK, Annemarie HALTER
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Patent number: 12610899Abstract: A spreading device, a powder-like mixture composition and a method tor spreading the composition for control or prevent forest pathogens on tree stumps are disclosed.Type: GrantFiled: July 26, 2022Date of Patent: April 28, 2026Assignee: Danstar Ferment AGInventors: Lauri Eskola, Pekka Seiskari
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Publication number: 20260103731Abstract: The present disclosure concerns a process for fermenting a biomass with a reduced dose of a purified exogenous enzyme (which can be, for example a purified exogenous glucoamylase). The process comprises contacting a biomass (which may comprise starch) with a recombinant yeast host cell. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having starch or dextrin hydrolase activity (which may be, for example, from a glucoamylase). The nucleic acid molecule encoding the heterologous polypeptide having starch or dextrin hydrolase activity comprises allowing the secretion of the heterologous polypeptide.Type: ApplicationFiled: December 11, 2025Publication date: April 16, 2026Applicant: DANSTAR FERMENT AGInventors: Aaron ARGYROS, Alexandra PANAITIU, Charles RICE, Matthew B. RICHARDS, Tera STOUGHTENGER, Kenneth BARRETT
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Patent number: 12593843Abstract: The present disclosure relates to a seed of a plant, a seedling or a plant derived from the seed containing an increased internal concentration of glycine-betaine compared to untreated controls. The present disclosure further relates to a method to produce a seed of a plant, a seedling or a plant derived from said seed containing an increased internal concentration of glycine-betaine which will confer to the seed, seedling or plant derived from said seed an enhanced germination potential and/or increased tolerance against various environmental stresses compared to untreated controls. The present disclosure further relates to uses of glycine-betaine for enhancing the germination potential of a seed and/or for increasing the tolerance of a seed against environmental stress. The present disclosure also relates to seedlings and plants, or parts thereof, derived from a seed of the present invention having an increased international concentration of glycine-betaine.Type: GrantFiled: December 7, 2018Date of Patent: April 7, 2026Assignee: DANSTAR FERMENT AGInventor: Olivier Cor
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Patent number: 12529079Abstract: The present disclosure concerns a process for fermenting a biomass with a reduced dose of a purified exogenous enzyme (which can be, for example a purified exogenous glucoamylase). The process comprises contacting a biomass (which may comprise starch) with a recombinant yeast host cell. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having starch or dextrin hydrolase activity (which may be, for example, from a glucoamylase). The nucleic acid molecule encoding the heterologous polypeptide having starch or dextrin hydrolase activity comprises allowing the secretion of the heterologous polypeptide.Type: GrantFiled: November 25, 2020Date of Patent: January 20, 2026Assignee: Danstar Ferment AGInventors: Aaron Argyros, Alexandra Panaitiu, Charles Rice, Matthew B. Richards, Tera Stoughtenger, Kenneth Barrett
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Patent number: 12522848Abstract: The present invention relates to host cells and their use wherein the host cells are capable of producing D-ribulose and incapable of or have a reduced capability of converting D-ribulose to a molecule other than ribitol, wherein the host cells comprise a heterologous nucleic acid sequence encoding a polypeptide capable of converting D-ribulose to ribitol with a cofactor preference for NADPH.Type: GrantFiled: December 17, 2020Date of Patent: January 13, 2026Assignee: DANSTAR FERMENT AGInventors: Astrid Schäfer, Yiming Chang, Sumire Honda Malca
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Patent number: 12448631Abstract: The present disclosure concerns a recombinant bacterial host cell capable of providing a nitrogen source to a yeast during fermentation to make ethanol. The recombinant bacterial host cell is capable of converting a biomass into ethanol. The recombinant bacterial host cell has at least one first genetic modification. The at least one genetic modifications confers to the recombinant bacterial host cell the ability to increase, when compared to a corresponding control bacterial cell lacking the at least one first genetic modification, the proteolytic activity associated with the recombinant bacterial host cell. The at least one genetic modification also confers the recombinant bacterial host cell the ability to provide a nitrogen source to a yeast capable of converting the biomass into ethanol, wherein the nitrogen source comprises a peptide, an amino acid and/or ammonia.Type: GrantFiled: July 9, 2021Date of Patent: October 21, 2025Assignee: DANSTAR FERMENT AGInventors: James L. Steele, Brooks Henningsen, Jeffery R. Broadbent, Ekkarat Phrommao, Fernanda Cristina Firmino
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Patent number: 12441976Abstract: The present disclosure provides an antimicrobial composition as well as a process using same to limit microbial activity in a yeast medium. The antimicrobial composition includes at least one weak acid, optionally in combination with an acid stable bacteriocin and/or an antibiotic. The antimicrobial composition can be used for propagating yeasts and for making a fermentation product. The present disclosure also provides yeasts and lactic acid bacteria that can be used with the antimicrobial composition.Type: GrantFiled: July 9, 2021Date of Patent: October 14, 2025Assignee: DANSTAR FERMENT AGInventors: Jeffery R. Broadbent, James L. Steele, Brooks Henningsen, Ekkarat Phrommao, Fernanda Cristina Firmino
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Patent number: 12442009Abstract: The present disclosure concerns the use of specific genetic modification(s) for improving sulfite tolerance in recombinant yeast host cells. The genetic modification(s) is (are) designed to allow the expression of an heterologous transcription factor favoring the expression of a SSU1 polypeptide and/or the expression of an heterologous SSU1 polypeptide in the recombinant yeast host cell(s).Type: GrantFiled: December 22, 2017Date of Patent: October 14, 2025Assignee: DANSTAR FERMENT AGInventors: Aaron Argyros, Charles F. Rice, Trisha Barrett, Michelle Oeser, Janet Fisher
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Patent number: 12428628Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.Type: GrantFiled: June 27, 2024Date of Patent: September 30, 2025Assignee: Danstar Ferment AGInventors: Ganesh M. Kishore, Michael Motion, Paula M. Hicks, Jorgen Hansen, Jens Houghton Larsen, Esben Halkjaer Hansen, Michael Dalgaard Mikkelsen, Sabina Tavares, Charlotte Blom
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Patent number: 12416034Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.Type: GrantFiled: April 7, 2023Date of Patent: September 16, 2025Assignee: Danstar Ferment AGInventors: Jens Houghton-Larsen, Paula Hicks, Michael Naesby, Thomas Tange Ostergaard, Jorgen Hansen, Michael Mikkelsen Dalgaard, Esben Hansen-Halkjaer, Ernesto Simon, Sabina Tavares
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Patent number: 12385052Abstract: The present disclosure provides yeasts, which can be recombinant yeast host cells, exhibiting prolonged persistence when submitted to a plurality of fermentation cycles. The yeasts exhibit at least one of the following phenotypic trait: a fast settling phenotype, a rugose phenotype, an improved invertase activity, triploidy, increased signaling in a RAS/cAMP/PKA pathway or combinations thereof.Type: GrantFiled: June 17, 2022Date of Patent: August 12, 2025Assignee: Danstar Ferment AGInventors: Emily Agnes Stonehouse, John McBride, Justin Van Rooyen, Zach Losordo, Petra Deane
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Patent number: 12365890Abstract: The present disclosure concerns yeast host cell especially suited for the expression of heterologous proteins, such as heterologous enzymes. The yeast host cell of the present disclosure exhibits an alteration in the cAMP signaling pathway which allows achieving increased heterologous protein yield and associated biological activity. The yeast host cell can also exhibit polyploidy.Type: GrantFiled: December 19, 2023Date of Patent: July 22, 2025Assignee: DANSTAR FERMENT AGInventors: Michelle Oeser, Janet Fisher, Aaron Argyros
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Patent number: 12344847Abstract: The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.Type: GrantFiled: March 13, 2018Date of Patent: July 1, 2025Assignee: DANSTAR FERMENT AGInventors: Aaron Argyros, Michelle Oeser, Erin Wiswall, Janet Fisher, Johannes Van Eijk, J. Kevin Kraus, Kevin Wenger, Brooks Henningsen, Ryan Skinner
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Patent number: 12258606Abstract: The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).Type: GrantFiled: March 14, 2023Date of Patent: March 25, 2025Assignee: DANSTAR FERMENT AGInventors: Charles F. Rice, Ryan Skinner, Trisha Barrett, Aaron Argyros
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Patent number: 12247208Abstract: The present disclosure concerns recombinant microbial host cell having, in glycolytic conditions, increased glycerol importing activity glycerol as well as, in high osmotic conditions, a decreased NAD-dependent glycerol-3-phosphate dehydrogenase (GPD) activity. The recombinant microbial host cell is particularly useful for the fermentation of sugarcane- or molasses-based medium for the production of ethanol.Type: GrantFiled: May 23, 2018Date of Patent: March 11, 2025Assignee: DANSTAR FERMENT AGInventors: Emily Agnes Stonehouse, John Evan Eck McBride, Kristen M. Deleault, Zachary Losordo, Mitchell Codd
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Publication number: 20250019681Abstract: The present invention relates to variants of an alpha-amylase which have an increased solubility at pH 6.0 compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in processing starch, cleaning or washing textiles, hard surfaces, or dishes, making ethanol, treating an oil well, processing pulp or paper, animal feed, syrup production, and preparing a dough or a baked product prepared from the dough.Type: ApplicationFiled: December 12, 2022Publication date: January 16, 2025Applicant: DANSTAR FERMENT AGInventors: Xuqiu Tan, Priya Anand, Asfia Qureshi
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Patent number: 12185725Abstract: The present invention generally relates to use of a biological control agent (BCA) in control of Septoria tritici blotch (STB) caused by Mycosphaerella graminicola. The BCA is, or comprises, Clonostachys rosea.Type: GrantFiled: December 18, 2018Date of Patent: January 7, 2025Assignee: DANSTAR FERMENT AGInventors: Dan Funck Jensen, Birgit Mikkelsen, Magnus Karlsson, Margareta Hökeberg
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Patent number: 12168768Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.Type: GrantFiled: October 13, 2021Date of Patent: December 17, 2024Assignees: DANSTAR FERMENT AG, STELLENBOSCH UNIVERSITYInventors: Elena Brevnova, John E. McBride, Erin Wiswall, Kevin S. Wenger, Nicky Caiazza, Heidi Hau, Aaron Argyros, Frank Agbogbo, Charles F. Rice, Trisha Barrett, John S. Bardsley, Abigail Foster, Anne K. Warner, Mark Mellon, Ryan Skinner, Indraneel Shikhare, Riaan Den Haan, Chhayal V. Gandhi, Alan Belcher, Vineet B. Rajgarhia, Allan C. Froehlich, Kristen M. Deleault, Emily Stonehouse, Shital A. Tripathi, Jennifer Gosselin, Yin-Ying Chiu, Haowen Xu
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Publication number: 20240373896Abstract: The present disclosure provides a yeast-based nutrients rich in sterols to prevent or reduce slow and/or sluggish alcoholic fermentation and maintaining or increase the fermenting capacity of Active Dry Yeast (ADY) or Instant Dry Yeast (IDY) during alcoholic fermentation. The present disclosure also provides a method of maintaining or increasing a fermenting capacity of Active Dry Yeast (ADY) or Instant Dry Yeast (IDY) during alcoholic fermentation.Type: ApplicationFiled: July 25, 2024Publication date: November 14, 2024Applicant: DANSTAR FERMENT AGInventors: Anne ORTIZ-JULIEN, David FERREIRA