Abstract: A pipette tip storage and dispensing system has a series of pipette tip holder cards each of which has a plurality of openings for holding a set of pipette tips extending partway through the openings in an upright orientation. The openings in each card match those in the other cards, and cards loaded with pipette tips can be stacked vertically with the pipette tips in one card extending into the open upper ends of the tips in the next adjacent card of the stack, to minimize storage space. An alignment device is arranged to hold the pipette tips in a card in a vertical orientation as the card is removed from the top of the stack and transferred to a pipette tip holder tray so that the pipette tips can be lowered into aligned openings in the holder tray with the card resting on the top wall of the tray.