Abstract: Method for flocculating microscopic particles in suspension in a liquid and application to the collection of phytoplankton microalgae and of zooplankton and for the purification of used water.One passes a first liquid comprising microscopic particles in suspension through a fixed granular layer (2) up to partial or complete clogging of said granular material by said microscopic particles; one unclogs at least partially said granular material in a fixed layer by passing a second liquid therethrough, moving in the same direction as the first liquid, but at a higher speed than the first; and one recovers below said layer flocculated aggregates of said microscopic particles and a liquid outflow (6).