Abstract: A method and primary pair and kit for the in vitro amplification of nucleic acid using amplification primers in amplification reactions. There is used a pair of primers comprising oligonucleotide sequences complementary to a part of a Direct Repeat sequence of a microorganism belonging to the M tuberculosis complex of microorganisms, whereby hybridization to a Direct Repeat occurs and subsequently elongation of the hybridized primer takes place. The primers are such that elongation in the amplification reaction occurs for one primer in a 5' direction and for the other primer in a 3' direction.
Type:
Grant
Filed:
January 22, 1997
Date of Patent:
June 13, 2000
Assignee:
De Staat der Nederlanden, Vertegenwoordigd door de Minister van Welzijn, Volksgezondhed en Cultuur
Inventors:
Johannes Dirk Anthonie Van Embden, Leendert Marinus Schouls, Judith Kamerbeek