Abstract: The Distributed Biofuel Manufacturing System (DBMS) integrates three critical innovations which may advance the state of the art for biofuels manufacturing to a distributed production and local distribution model. This model yields a sustainable and commercially viable means of production for biofuels. Essential to this new model is a Portable Biorefinery (PBR) which allows for onsite conversion of biomass into biofuels, an accompanying set of advanced biocatalysts, and finally a multi-role harvesting and pre-processing technology to optimize both deliberate and “threat” biomass for biofuels. Utilizing either high yield deliberate biomass “plantations” or salvaging available threat biomass, the portable biorefinery will process high volumes of variable biomass into dense liquid energy format and other energy products which can then be utilized locally. This disclosure seeks to avoid the logistical overhead and economic limitations of traditional centralized industrial fuels manufacturing.