Abstract: Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs an EPSP synthase gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide glyphosate. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen. This invention also relates to methods of producing human or animal food from the transformed maize plants.

Abstract: The invention provides genetically engineered, preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of at least one amino acid essential to the diet of animals, or increasing the starch content, of a plant. One such method involves stably transforming a cell of a plant with an a preselected DNA sequence encoding an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, preferably a seed storage protein which is deficient in at least one amino acid essential to the diet of animals. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I015011. This invention thus relates to the plants, seeds and tissue cultures of the variety I015011, and to methods for producing a corn plant produced by crossing a corn plant of variety I015011 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I015011 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I015011, and also to the SSR and isozyme typing profiles of corn variety I015011.

Abstract: The invention provides isolated gamma coixin promoters useful for the expression of heterologous coding sequences in transgenic plants. Further provided by the invention are transgenic plants comprising such promoters, expression constructs for the introduction of heterologous coding sequences into plants, and methods of using the plants and nucleic acids provided by the invention.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I026458. This invention thus relates to the plants, seeds and tissue cultures of the variety I026458, and to methods for producing a corn plant produced by crossing a corn plant of variety I026458 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I026458 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I026458, and also to the SSR and isozyme typing profiles of corn variety I026458.

Abstract: According to the invention, there is provided an inbred corn plant designated LIZL5. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant LIZL5, and to methods for producing a corn plant produced by crossing the inbred corn plant LIZL5 with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant LIZL5 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant LIZL5, and also to the SSR and genetic isozyme typing profiles of inbred corn plant LIZL5. This invention further relates to methods of transforming the inbred LIZL5 and cells thereof, transformed plants produced by these methods, progeny transgenic plants, and seed derived therefrom.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I180580. This invention thus relates to the plants, seeds and tissue cultures of the variety I180580, and to methods for producing a corn plant produced by crossing a corn plant of variety I180580 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I180580 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I180580, and also to the SSR and isozyme typing profiles of corn variety I180580.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I015036. This invention thus relates to the plants, seeds and tissue cultures of the variety I015036, and to methods for producing a corn plant produced by crossing a corn plant of variety I015036 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I015036 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I015036, and also to the SSR and isozyme typing profiles of corn variety I015036.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I450436. This invention thus relates to the plants, seeds and tissue cultures of the variety I450436, and to methods for producing a corn plant produced by crossing a corn plant of variety I450436 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I450436 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I450436, and also to the SSR and isozyme typing profiles of corn variety I450436.

Abstract: The invention provides preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of an amino acid essential to the diet of animals, or increasing the starch content, of a plant. One method involves stably transforming a cell of a plant with a preselected DNA sequence encoding an RNA molecule substantially identical or complementary to a messenger RNA encoding a plant seed storage protein. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide. The transformed cells are used to generate fertile transgenic plants and seeds, which are characterized by expression of the preselected DNA sequence.

Abstract: Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminceous cereal plants which have not proven stably transformable by other techniques.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I244225. This invention thus relates to the plants, seeds and tissue cultures of the variety I244225, and to methods for producing a corn plant produced by crossing a corn plant of variety I244225 with itself or with another corn plant, such as a plant of another variety. This invention furthers relates to corn seeds and plants produced by crossing plants of variety I244225 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I244225, and also to the SSR and isozyme typing profiles of corn variety I244225.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I363128. This invention thus relates to the plants, seeds and tissue cultures of the variety I363128, and to methods for producing a corn plant produced by crossing a corn plant of variety I363128 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I363128 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I363128, and also to the SSR and isozyme typing profiles of corn variety I363128.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated I501150. This invention thus relates to the plants, seeds and tissue cultures of the variety I501150, and to methods for producing a corn plant produced by crossing a corn plant of variety I501150 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I501150 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I501150, and also to the SSR and isozyme typing profiles of corn variety I501150.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: The invention is directed to a method of breeding maize using female plants having a transgene encoding an EPSPS enzyme where the female plants are female reproductively tolerant to treatment with glyphosate but can be made male sterile upon treatment with glyphosate.