Patents Assigned to Denka Seiken Co., Ltd.
  • Patent number: 10883985
    Abstract: Disclosed is an immunochromatographic test strip by which the influences by the interfering substances in the immunochromatography method, contained in test samples, are reduced, so that it enables to accurately and specifically measure a test substance in a test sample irrespective of the amount of the test sample supplied to the assay, and to provide an immunochromatography method using the immunochromatographic test strip. The immunochromatographic test strip includes, in the order from upstream, a sample pad, a labeled substance region, a detection region and an absorption band, wherein a polymer(s) in which hydrophobic cyclic monomer(s) having an ionic functional group(s) is/are polymerized is impregnated at a region(s) upstream of the labeled substance region.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: January 5, 2021
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Risa Kohiyama, Osamu Ishikawa, Yuki Shinohara, Takashi Miyazawa
  • Publication number: 20200318155
    Abstract: Disclosed are a method and reagent of quantifying cholesterol in triglyceride-rich lipoprotein (TRL-C) in a test sample in a more specific manner without requiring laborious operations. The method of quantifying cholesterol in triglyceride-rich lipoprotein (TRL-C) includes the steps of: (1) selectively eliminating cholesterol in lipoproteins other than triglyceride-rich lipoprotein (TRL) by allowing a cholesterol esterase having a molecular weight of more than 50 kDa and a surfactant(s) to act; and (2) quantifying the remaining TRL-C.
    Type: Application
    Filed: May 23, 2017
    Publication date: October 8, 2020
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Noriyuki SATOH, Makoto IKAIDA, Yuhko HIRAO, Yasuki ITOH
  • Publication number: 20200124604
    Abstract: Provided is a biomarker for detecting colorectal cancer in an early stage. A colorectal cancer biomarker for detecting colorectal cancer, wherein the biomarker consists of at least one protein of the following 22 proteins with numbers 1 to 22, or at least one peptide of the partial peptides of the proteins with numbers 1 to 22: 1. Annexin A11; 2. Annexin A3; 3. Annexin A4; 4. Tanascin-N; 5. Transferrin receptor protein 1; 6. Glucose transporter 1; 7. Complement component C9; 8. CD88 antigen; 9. 78 kDa glucose-regulated protein; 10. ?-1-acid glycoprotein; 11. Matrix metalloproteinase-9; 12. Angiopoietin-1; 13. CD67 antigen; 14. Mucin-5B; 15. Adapter protein GRB2; 16. Annexin A5; 17. Olfactomedin-4; 18. Neutral amino acid transporter B(0); 19. Tripeptidyl-peptidase 1; 20. Heat shock-related 70 kDa protein 2; 21. Proteasome subunit ? type-5; or 22. Neutrophil gelatinase-associated lipocalin.
    Type: Application
    Filed: June 29, 2018
    Publication date: April 23, 2020
    Applicants: National Institutes of Biomedical Innovation, Health and Nutrition, DENKA SEIKEN CO., LTD.
    Inventors: Takeshi Tomonaga, Takashi SHIROMIZU
  • Publication number: 20200049703
    Abstract: It is intended to provide an immunochromatographic test piece which prevents a non-specific reaction by efficiently and continuously contacting and neutralizing a developing solution containing nitrous acid with a neutralizing reagent in an immunochromatography method of extracting and measuring a sugar chain antigen by nitrous acid extraction on the immunochromatographic test piece.
    Type: Application
    Filed: March 14, 2018
    Publication date: February 13, 2020
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Daisuke KATO, Shino MURAMATSU, Tomohiro HATTORI
  • Publication number: 20200038854
    Abstract: It is an object of the present invention to provide a method or an immunochromatographic test piece, which controls a speed or a direction of development of a specimen on the immunochromatographic test piece, so that a treatment with an acid reagent, nitrite, and a neutralizing reagent is properly controlled.
    Type: Application
    Filed: March 4, 2018
    Publication date: February 6, 2020
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Daisuke KATO, Shino MURAMATSU, Tomohiro HATTORI
  • Patent number: 10527618
    Abstract: Means for enabling an immunoassay with a sufficient sensitivity in an immunoassay for measuring influenza virus in a sample using influenza virus M1 protein as an antigen is provided. A sample processing method in an immunoassay for influenza virus, which method comprises, in an immunoassay for influenza virus using an antibody which undergoes antigen-antibody reaction with influenza virus matrix 1 protein, or an antigen-binding fragment thereof, bringing a sample containing influenza virus into contact with a sample processing liquid containing a surfactant having at least one group selected from the group consisting of palmityl, stearyl, and oleyl, is provided.
    Type: Grant
    Filed: September 10, 2014
    Date of Patent: January 7, 2020
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Yuki Shinohara, Takashi Miyazawa
  • Publication number: 20190361029
    Abstract: The object of the present invention is to provide insoluble carrier particles used for high-sensitivity rapid immunoassay, which allow visual observation and high-sensitivity apparatus measurement. The insoluble carrier particles are visible colored insoluble carrier particles labeled with a fluorescent dye, which are used for immunoassay, wherein absorption of fluorescence by the visible colored insoluble carrier particles is low within a wavelength range of fluorescence emitted by the fluorescent dye.
    Type: Application
    Filed: August 2, 2019
    Publication date: November 28, 2019
    Applicant: Denka Seiken Co., Ltd.
    Inventor: Takashi Miyazawa
  • Patent number: 10436783
    Abstract: This invention provides a method for easily collecting antigens possessed by microorganisms without the use of special equipment. The method for collecting microbial antigens comprises: allowing a specimen containing microorganisms to pass through a filter membrane with a pore diameter that does not allow microorganisms to pass therethrough; capturing the microorganisms in the specimen on the filter membrane; applying a microbial destruction reagent capable of microbial membrane destruction to the filter membrane comprising the microorganisms captured thereon to destruct the captured microorganisms on the filter membrane; and collecting antigens in the filtrate.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: October 8, 2019
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Yuji Saito, Daisuke Kato
  • Patent number: 10415077
    Abstract: Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.
    Type: Grant
    Filed: September 11, 2008
    Date of Patent: September 17, 2019
    Assignee: DENKA SEIKEN CO., LTD.
    Inventor: Yuhko Hirao
  • Publication number: 20190277838
    Abstract: The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other.
    Type: Application
    Filed: May 23, 2019
    Publication date: September 12, 2019
    Applicant: Denka Seiken Co., Ltd.
    Inventors: Risa Kohiyama, Osamu ISHIKAWA, Takashi MIYAZAWA
  • Patent number: 10365277
    Abstract: Disclosed is a novel method for measuring haemagglutinin of an influenza virus, which can construct an assay system in a shorter period of time than a sandwich immunoassay method using two kinds of anti-haemagglutinin antibodies. The method for measuring haemagglutinin of an influenza virus is achieved by a sandwich immunoassay method comprising sandwiching the haemagglutinin between a lectin which binds to the haemagglutinin but does not bind to an antibody, and an anti-haemagglutinin antibody which undergoes antigen-antibody reaction with the haemagglutinin.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: July 30, 2019
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Ryotaro Mitsumata, Noriyuki Izutani
  • Publication number: 20190226952
    Abstract: Methods of agglutinating and separating erythrocytes, by which erythrocytes can be instantaneously agglutinated into a sufficient size in a blood sample and completely separated from the blood sample; and a hemagglutination reagent are provided. The method of agglutinating erythrocytes according to the present invention includes adding a solution containing a cholic acid-based surfactant and an acid to a blood sample. The method of separating erythrocytes according to the present invention includes separating the erythrocytes agglutinated by the above-described method of the present invention. The hemagglutination reagent according to the present invention contains a cholic acid-based surfactant and an acid.
    Type: Application
    Filed: October 4, 2017
    Publication date: July 25, 2019
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Shino MURAMATSU, Daisuke KATO, Tomohiro HATTORI
  • Patent number: 10345294
    Abstract: The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: July 9, 2019
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Risa Kohiyama, Osamu Ishikawa, Takashi Miyazawa
  • Publication number: 20190154711
    Abstract: A method that enables quantification of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring a laborious operation is disclosed. The method for quantifying cholesterol in HDL3 comprises: Step 1 wherein phospholipase and/or sphingomyelinase is/are allowed to act on a test sample to transfer cholesterol to the outside of the reaction system; and Step 2 wherein cholesterol remaining in the reaction system is quantified. The method enables specific quantification of HDL3 cholesterol in a test sample using an automatic analyzer without requirement of a laborious operation such as ultracentrifugation or pretreatment. Further, quantification of the HDL2 cholesterol level can also be carried out by subtracting the HDL3 cholesterol level from the total HDL cholesterol level obtained by a conventional method for quantifying the total HDL cholesterol in a test sample.
    Type: Application
    Filed: January 17, 2019
    Publication date: May 23, 2019
    Applicant: Denka Seiken Co., Ltd.
    Inventors: Maiko HIGUCHI, Yasuki ITOH
  • Patent number: 10295533
    Abstract: The present invention provides a test kit capable of rapid and highly sensitive detection. The test kit is provided with a first member, which contains a region in which is held a labeling substance with a label immobilized on a substance that specifically binds with a substance to be detected, and a second member, which has a detection zone where the labeling substance is captured through the substance to be detected, which is connected downstream of the first member in the developing direction, and which allows the labeling substance contained in a liquid sample that flows in from the first member, to develop into the detection zone.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: May 21, 2019
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Takashi Miyazawa, Yuki Shinohara
  • Publication number: 20190145864
    Abstract: The present invention relates to an immunochromatography test method of measuring a sugar chain antigen, which provides an immunochromatographic test piece and a specimen adding device capable of specifically measuring a sugar chain antigen, and an immunochromatography method using the same; wherein after mixing a specimen with a nitrite solution, a step of allowing tartaric acid to come into contact with the mixture, and extracting a sugar chain antigen contained in the specimen is carried out in a filtration step.
    Type: Application
    Filed: June 8, 2017
    Publication date: May 16, 2019
    Applicant: DENKA SEIKEN CO., LTD
    Inventors: Daisuke Kato, Tomohiro HATTORI, Shino MURAMATSU
  • Publication number: 20190101532
    Abstract: Disclosed are an immunochromatographic test device which allows a target substance to be detected or quantified more rapidly and sensitively than conventional methods, and a method of forming a sample addition part of the test device. The method of forming a sample addition part of the immunochromatographic test device includes applying a surfactant that is in white powder form under normal conditions, such as sodium deoxycholate, to the sample addition part and drying it. The immunochromatographic test device includes the sample addition part formed by the method.
    Type: Application
    Filed: March 23, 2016
    Publication date: April 4, 2019
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Yuki SHINOHARA, Miwa KUWAHARA, Hiroshi AKITA, Takashi MIYAZAWA
  • Publication number: 20180306823
    Abstract: Disclosed is a method for quantifying HDL2 cholesterol in a test sample without requiring laborious operations. The method for quantifying cholesterol comprises allowing phospholipase to act on HDL to quantify cholesterol. Also disclosed is a method comprising: a first step of transferring cholesterols other than high-density lipoproteins in a test sample to the outside of the reaction system; and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system; wherein, by performing the second step by the above method, high-density lipoprotein 2 cholesterol in the test sample can be quantified.
    Type: Application
    Filed: June 26, 2018
    Publication date: October 25, 2018
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Maiko HIGUCHI, Yasuki ITOH
  • Patent number: 10077286
    Abstract: Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: September 18, 2018
    Assignee: DENKA SEIKEN CO., LTD.
    Inventor: Motoko Ohta
  • Patent number: 10031145
    Abstract: Disclosed is a method for quantifying HDL2 cholesterol in a test sample without requiring laborious operations. The method for quantifying cholesterol comprises allowing phospholipase to act on HDL to quantify cholesterol. Also disclosed is a method comprising: a first step of transferring cholesterols other than high-density lipoproteins in a test sample to the outside of the reaction system; and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system; wherein, by performing the second step by the above method, high-density lipoprotein 2 cholesterol in the test sample can be quantified.
    Type: Grant
    Filed: February 27, 2012
    Date of Patent: July 24, 2018
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Maiko Higuchi, Yasuki Itoh