Patents Assigned to Denka Seiken Co., Ltd.
  • Patent number: 10415077
    Abstract: Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.
    Type: Grant
    Filed: September 11, 2008
    Date of Patent: September 17, 2019
    Assignee: DENKA SEIKEN CO., LTD.
    Inventor: Yuhko Hirao
  • Publication number: 20190277838
    Abstract: The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other.
    Type: Application
    Filed: May 23, 2019
    Publication date: September 12, 2019
    Applicant: Denka Seiken Co., Ltd.
    Inventors: Risa Kohiyama, Osamu ISHIKAWA, Takashi MIYAZAWA
  • Patent number: 10365277
    Abstract: Disclosed is a novel method for measuring haemagglutinin of an influenza virus, which can construct an assay system in a shorter period of time than a sandwich immunoassay method using two kinds of anti-haemagglutinin antibodies. The method for measuring haemagglutinin of an influenza virus is achieved by a sandwich immunoassay method comprising sandwiching the haemagglutinin between a lectin which binds to the haemagglutinin but does not bind to an antibody, and an anti-haemagglutinin antibody which undergoes antigen-antibody reaction with the haemagglutinin.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: July 30, 2019
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Ryotaro Mitsumata, Noriyuki Izutani
  • Publication number: 20190226952
    Abstract: Methods of agglutinating and separating erythrocytes, by which erythrocytes can be instantaneously agglutinated into a sufficient size in a blood sample and completely separated from the blood sample; and a hemagglutination reagent are provided. The method of agglutinating erythrocytes according to the present invention includes adding a solution containing a cholic acid-based surfactant and an acid to a blood sample. The method of separating erythrocytes according to the present invention includes separating the erythrocytes agglutinated by the above-described method of the present invention. The hemagglutination reagent according to the present invention contains a cholic acid-based surfactant and an acid.
    Type: Application
    Filed: October 4, 2017
    Publication date: July 25, 2019
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Shino MURAMATSU, Daisuke KATO, Tomohiro HATTORI
  • Patent number: 10345294
    Abstract: The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: July 9, 2019
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Risa Kohiyama, Osamu Ishikawa, Takashi Miyazawa
  • Publication number: 20190154711
    Abstract: A method that enables quantification of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring a laborious operation is disclosed. The method for quantifying cholesterol in HDL3 comprises: Step 1 wherein phospholipase and/or sphingomyelinase is/are allowed to act on a test sample to transfer cholesterol to the outside of the reaction system; and Step 2 wherein cholesterol remaining in the reaction system is quantified. The method enables specific quantification of HDL3 cholesterol in a test sample using an automatic analyzer without requirement of a laborious operation such as ultracentrifugation or pretreatment. Further, quantification of the HDL2 cholesterol level can also be carried out by subtracting the HDL3 cholesterol level from the total HDL cholesterol level obtained by a conventional method for quantifying the total HDL cholesterol in a test sample.
    Type: Application
    Filed: January 17, 2019
    Publication date: May 23, 2019
    Applicant: Denka Seiken Co., Ltd.
    Inventors: Maiko HIGUCHI, Yasuki ITOH
  • Patent number: 10295533
    Abstract: The present invention provides a test kit capable of rapid and highly sensitive detection. The test kit is provided with a first member, which contains a region in which is held a labeling substance with a label immobilized on a substance that specifically binds with a substance to be detected, and a second member, which has a detection zone where the labeling substance is captured through the substance to be detected, which is connected downstream of the first member in the developing direction, and which allows the labeling substance contained in a liquid sample that flows in from the first member, to develop into the detection zone.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: May 21, 2019
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Takashi Miyazawa, Yuki Shinohara
  • Publication number: 20190145864
    Abstract: The present invention relates to an immunochromatography test method of measuring a sugar chain antigen, which provides an immunochromatographic test piece and a specimen adding device capable of specifically measuring a sugar chain antigen, and an immunochromatography method using the same; wherein after mixing a specimen with a nitrite solution, a step of allowing tartaric acid to come into contact with the mixture, and extracting a sugar chain antigen contained in the specimen is carried out in a filtration step.
    Type: Application
    Filed: June 8, 2017
    Publication date: May 16, 2019
    Applicant: DENKA SEIKEN CO., LTD
    Inventors: Daisuke Kato, Tomohiro HATTORI, Shino MURAMATSU
  • Publication number: 20190101532
    Abstract: Disclosed are an immunochromatographic test device which allows a target substance to be detected or quantified more rapidly and sensitively than conventional methods, and a method of forming a sample addition part of the test device. The method of forming a sample addition part of the immunochromatographic test device includes applying a surfactant that is in white powder form under normal conditions, such as sodium deoxycholate, to the sample addition part and drying it. The immunochromatographic test device includes the sample addition part formed by the method.
    Type: Application
    Filed: March 23, 2016
    Publication date: April 4, 2019
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Yuki SHINOHARA, Miwa KUWAHARA, Hiroshi AKITA, Takashi MIYAZAWA
  • Publication number: 20180306823
    Abstract: Disclosed is a method for quantifying HDL2 cholesterol in a test sample without requiring laborious operations. The method for quantifying cholesterol comprises allowing phospholipase to act on HDL to quantify cholesterol. Also disclosed is a method comprising: a first step of transferring cholesterols other than high-density lipoproteins in a test sample to the outside of the reaction system; and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system; wherein, by performing the second step by the above method, high-density lipoprotein 2 cholesterol in the test sample can be quantified.
    Type: Application
    Filed: June 26, 2018
    Publication date: October 25, 2018
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Maiko HIGUCHI, Yasuki ITOH
  • Patent number: 10077286
    Abstract: Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: September 18, 2018
    Assignee: DENKA SEIKEN CO., LTD.
    Inventor: Motoko Ohta
  • Patent number: 10031145
    Abstract: Disclosed is a method for quantifying HDL2 cholesterol in a test sample without requiring laborious operations. The method for quantifying cholesterol comprises allowing phospholipase to act on HDL to quantify cholesterol. Also disclosed is a method comprising: a first step of transferring cholesterols other than high-density lipoproteins in a test sample to the outside of the reaction system; and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system; wherein, by performing the second step by the above method, high-density lipoprotein 2 cholesterol in the test sample can be quantified.
    Type: Grant
    Filed: February 27, 2012
    Date of Patent: July 24, 2018
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Maiko Higuchi, Yasuki Itoh
  • Patent number: 10018641
    Abstract: Disclosed is the provision of a method for quantifying HDL3 in a test sample without requiring a laborious operation. The method for quantifying cholesterol in high-density lipoprotein 3 comprises allowing a surfactant(s) which specifically react(s) with a high-density lipoprotein 3 to react with a test sample and quantifying cholesterol, and the surfactant(s) is(are) at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether.
    Type: Grant
    Filed: January 24, 2013
    Date of Patent: July 10, 2018
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Noriyuki Sato, Yuhko Hirao, Yasuki Itoh
  • Publication number: 20180172682
    Abstract: Disclosed is an immunochromatographic test strip by which the influences by the interfering substances in the immunochromatography method, contained in test samples, are reduced, so that it enables to accurately and specifically measure a test substance in a test sample irrespective of the amount of the test sample supplied to the assay, and to provide an immunochromatography method using the immunochromatographic test strip. The immunochromatographic test strip includes, in the order from upstream, a sample pad, a labeled substance region, a detection region and an absorption band, wherein a polymer(s) in which hydrophobic cyclic monomer(s) having an ionic functional group(s) is/are polymerized is impregnated at a region(s) upstream of the labeled substance region.
    Type: Application
    Filed: May 20, 2016
    Publication date: June 21, 2018
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Risa KOHIYAMA, Osamu ISHIKAWA, Yuki SHINOHARA, Takashi MIYAZAWA
  • Publication number: 20180164316
    Abstract: Disclosed is a novel method of detecting a test subject by an immunoassay, in which low sensitivity due to using a monoclonal antibody is improved in spite of using a monoclonal antibody in the immunoassay. The method of detecting a test subject is a method of detecting a test subject having two or more kinds of antigens which enable detection of said test subject, which method is performed by an immunoassay such as a sandwich method, using a monoclonal antibody or an antigen-binding fragment thereof which recognizes the two or more kinds of antigens.
    Type: Application
    Filed: May 27, 2016
    Publication date: June 14, 2018
    Applicant: DENKA SEIKEN CO., LTD.
    Inventors: Risa KOHIYAMA, Takashi MIYAZAWA
  • Publication number: 20180120324
    Abstract: The present invention relates to a method of estimating the number of urinary podocytes, including detecting podocalyxin in a urinary sediment sample liquid, and more specifically, to a method of estimating the number of urinary podocytes, including the following steps (1) to (3): (1) a step of preparing the urinary sediment sample liquid by separating urinary sediment from urine collected from a test subject and solubilizing podocalyxin in the urinary sediment; (2) a step of calculating a podocalyxin excretion amount in the urinary sediment sample liquid through detection of podocalyxin in the urinary sediment sample liquid; and (3) a step of calculating the number of urinary podocytes by dividing the podocalyxin excretion amount in the urinary sediment sample liquid by a podocalyxin amount per podocyte.
    Type: Application
    Filed: April 21, 2016
    Publication date: May 3, 2018
    Applicants: Denka Company Limited, Denka Seiken Co., Ltd.
    Inventors: Masanori Hara, Hiroyuki Kurosawa, Yoshiaki Hirayama
  • Patent number: 9939436
    Abstract: Disclosed are an immunoassay method which can measure an antigen with high sensitivity and accuracy; and a reagent therefor. In the immunoassay method, an antigen-antibody reaction and/or a measurement is(are) carried out in the presence of a polycarboxylic acid type surfactant. The immunoassay reagent for use in the method is characterized by comprising the polycarboxylic acid type surfactant. By employing such a simple means that the polycarboxylic acid type surfactant is allowed to be present in the reaction and/or measurement system, non-specific reactions can be suppressed effectively even in a highly sensitive immunoassay, and an antigen can be measured accurately and specificity can be improved in the immunoassay.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: April 10, 2018
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Mayumi Kano, Ritsuko Tachibana, Masayuki Iizuka
  • Publication number: 20180095088
    Abstract: An object of the present invention is to improve visibility by reducing background noise in order to accurately detect the signal from a target substance in an immunochromatographic device. The immunochromatographic device comprises a membrane having a detection region, to which an antibody or antigen serving as a capturing substance capable of capturing a target substance is immobilized, for detecting the target substance by using an antigen or antibody labeled with a labeling carrier that is a colored particle to form a complex of the capturing substance-target substance-labeled antigen or antibody in the capturing substance-immobilized detection region on the device, based on color of the labeling carrier, wherein a colorant having a color complementary to the color of the labeling carrier is allowed to be contained in a dry state in a constituent member of the device such that the colorant is developed together with a specimen when the specimen is developed on the device.
    Type: Application
    Filed: April 27, 2016
    Publication date: April 5, 2018
    Applicant: DENKA SEIKEN CO., LTD
    Inventors: Osamu Ishikawa, Risa KOHIYAMA, Takashi MIYAZAWA
  • Patent number: 9897601
    Abstract: The present invention provides a test kit capable of rapid and highly sensitive detection.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: February 20, 2018
    Assignee: Denka Seiken Co., Ltd
    Inventors: Takashi Miyazawa, Yuki Shinohara
  • Patent number: 9890412
    Abstract: Provided is a method for separately or simultaneously quantifying whole HDL-C and cholesterol in HDL subfractions: ApoE-Containing HDL-C and ApoE-deficient HDL-C. A method for enzymatically and separately quantifying cholesterol in the ApoE-deficient HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative to a test sample in a final concentration of 0.05 to 0.10%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated. A method for enzymatically and separately quantifying cholesterol in ApoE-Containing HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative so as to obtain a final concentration of 0.15 to 0.75%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated.
    Type: Grant
    Filed: April 10, 2013
    Date of Patent: February 13, 2018
    Assignees: Denka Seiken Co., Ltd., National University Corporation Hokkaido University
    Inventors: Yasuki Itoh, Hitoshi Chiba