Abstract: A method of graphically representing mathematical expressions in both audio and visual formats on a user device is described. Embodiments of the present invention include an Internet-enabled audio-visual graphing calculator that receives input from a user device in at least one of at least one of audio, visual, or Braille formats. An embodiment of the present invention interprets input received from the user device as a typeset mathematical expression, parses the typeset mathematical expression into an interpreted mathematical expression and compiles the interpreted mathematical expression into an evaluation function. At least one point is sampled on the evaluation function. The sampled evaluation function is rendered as a graph in a tactile graphic using a Braille display or embosser, or on a visual display. In an embodiment of the invention, an audible representation of the rendered graph is generated for playback on the user device.

Abstract: A method of graphically representing mathematical expressions in both audio and visual formats on a user device is described. Embodiments of the present invention include an Internet-enabled audio-visual graphing calculator that receives input from a user device in at least one of at least one of audio, visual, or Braille formats. An embodiment of the present invention interprets input received from the user device as a typeset mathematical expression, parses the typeset mathematical expression into an interpreted mathematical expression and compiles the interpreted mathematical expression into an evaluation function. At least one point is sampled on the evaluation function. The sampled evaluation function is rendered as a graph on a visual display of a user device. In an embodiment of the invention, an audible representation of the rendered graph is generated for playback on the user device.

Abstract: A method of graphically representing mathematical expressions in both audio and visual formats on a user device is described. Embodiments of the present invention include an Internet-enabled audio-visual graphing calculator that receives input from a user device in at least one of at least one of audio, visual, or Braille formats. An embodiment of the present invention interprets input received from the user device as a typeset mathematical expression, parses the typeset mathematical expression into an interpreted mathematical expression and compiles the interpreted mathematical expression into an evaluation function. At least one point is sampled on the evaluation function. The sampled evaluation function is rendered as a graph on a visual display of a user device. In an embodiment of the invention, an audible representation of the rendered graph is generated for playback on the user device.

Abstract: A method of graphically representing mathematical expressions in both audio and visual formats on a user device is described. Embodiments of the present invention include an Internet-enabled audio-visual graphing calculator that receives input from a user device in at least one of at least one of audio, visual, or Braille formats. An embodiment of the present invention interprets input received from the user device as a typeset mathematical expression, parses the typeset mathematical expression into an interpreted mathematical expression and compiles the interpreted mathematical expression into an evaluation function. At least one point is sampled on the evaluation function. The sampled evaluation function is rendered as a graph on a visual display of a user device. In an embodiment of the invention, an audible representation of the rendered graph is generated for playback on the user device.

Abstract: A method for purifying soluble laminin 5 from conditioned cell culture medium. A nonionic or anionic detergent is added to conditioned medium to a final concentration of between 0.1% and 1.0%. The conditioned medium is purified by cation exchange chromatography and anion exchange chromatography, yielding laminin 5 of at least about 70% purity.

Abstract: A method of increasing the number of adult pancreatic islet cells available for transplantation by contacting the cells with laminin 5 extracellular matrix. When contacted with the deposited matrix produced by 804G rat bladder carcinoma cells, a 1,500 fold increase in cell number is observed after three passages in culture. Islet cell expansion also occurs when cells are contacted with 804G soluble matrix. The expanded islet cells contain insulin and respond to glucose challenge.

Abstract: A method of expanding the number of pancreatic islet cells for transplantation. Fetal islet cells are cultured in the presence of laminin 5 extracellular matrix, resulting in a significant increase in cell number after passaging in culture. The expanded islet cells contain insulin and respond to glucose challenge.

Abstract: A method for growing epithelial cells in vitro using soluble proteins secreted by 804G and NBT-II rat bladder carcinoma cells. These proteins stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The protein is purified from conditioned medium by immunoaffinity chromatography using a monoclonal antibody generated against the protein. The purification of these proteins may be facilitated by culturing the cells under low serum or serum free conditions.

Abstract: A trans-epithelial appliance having a hemidesmosome formation-inducing protein composition derived from rat bladder carcinoma cells deposited thereon. This composition stimulates cell attachment and may be either the cell matrix or a soluble factor isolated from the conditioned medium. The appliance will be useful for diminishing inflammation and/or infection at the site of entry of the appliance. The appliance may also be used to stimulate gum junctional epithelium adhesion in the treatment of gingivitis and periodontitis. The composition may be used to maintain tissues ex vivo.

Abstract: A method for growing epithelial cells in vitro is described, The matrix secreted by 804G rat bladder carcinoma cells is able to stimulate cell attachment and hemidesmosome formation in cells grown on the matrix, Human epithelial cells grown on the 804G matrix are able to produce normal hemidesmosomes and attach to their growing substrate.

Abstract: A method for growing endocrine precursor cells in vitro, such as pancreatic islet precursors, by culturing such cells in the presence or absence of cell matrix proteins capable of promoting hemidesmosome formation, such as those produced by the rat bladder carcinoma cell line 804G.

Abstract: A method for growing epithelial cells in vitro using soluble proteins secreted by 804G rat bladder carcinoma cells. These proteins are able to stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The purification of these proteins from 804G culture supernatant is greatly facilitated by culturing the cells under low serum conditions.