Abstract: A semi-automatic medical continuous-firing clip applier includes a grip and a forceps rod, one end of the forceps rod is connected to the grip, and the other end of the forceps rod is connected to an upper jaw and a lower jaw; the upper jaw and the lower jaw are connected to a trigger, where the forceps rod is internally provided with a biological clip cartridge; the upper jaw and the lower jaw are provided with biological clip positioning grooves; the forceps rod is further internally provided with a transmission mechanism respectively in coordination with the biological clip cartridge and a biological clip propelling apparatus. The biological clip propelling apparatus is configured to drive a biological clip in the biological clip cartridge to enter the positioning grooves in the upper and lower jaws, and then the trigger is tightly held to close the biological clip.

Abstract: A method of introducing cytoplasmically inherited traits from a donor plant into a receptor plant of the Solanaceae family has been invented. Protoplasts from the donor and receptor plants are prepared. The donor protoplasts are treated under suitable conditions to prevent extensive nuclear divisions. The treated donor protoplasts and receptor protoplasts are then contacted under suitable conditions permitting the fusion of the protoplasts. Plantlets are regenerated from the fused protoplasts, and plantlets which contain the donor cytoplasmic traits are selected and rooted. Plantlets which contain receptor cytoplasmic traits are discarded.