Abstract: A method and kit for selective amplification of desired amplicons in a two-stage polymerase chain reaction is provided. The method and kit uses target primers comprising mismatch control sequences; wherein any pair of primers that flank a desired amplicon sequence have non-matching mismatch control sequences. and any pair of primers that flank an undesired amplicon sequence have matching mismatch control sequences.
Type:
Grant
Filed:
January 27, 2017
Date of Patent:
April 2, 2019
Assignee:
Devyser Holding AB
Inventors:
Ulf Klangby, Anders Hedrum, Steffen Heim