Abstract: The present invention is related to a Template Switch Oligo construct and its use into a ligase free diagnostic and/or sequencing method and to the kit for performing the method of the invention.

Abstract: The present invention is related to a Template Switch Oligo construct and its use into a ligase free diagnostic and/or sequencing method and to the kit for performing the method of the invention.

Abstract: A high throughput sequencing method and kit The present invention is in the field of diagnostic and sequencing technologies and is related to a high throughput sequencing method and a kit comprising tools for performing this method, that combine a capture and amplification by switching detection step, preferably the so-called “Capture and Amplification by Tailing and Switching” (CATS) and sequencing technology, preferably the so-called “Nanoballs sequencing” technologies.

Abstract: Assembly kit, comprising a sonication device (10) and a support (20) for sample tubes (30). The device comprises a tank (13) and an ultrasound wave generator (15) coupled to the tank and designed to generate ultrasound waves having a frequency of between 20 kHz and 80 kHz. The tank comprises an indicator (132) of a level (133) of liquid. The support for sample tubes comprises a means (21) for the support of several sample tubes of a size of 0.2 ml, a rod (22) secured to the support means, and a means (23) for suspending the rod in the tank. The rod has a length such that, in the assembly position, the sample tubes (30) are positioned with their lower end between 8 mm and 12 mm below the indicator (132) of the level of liquid.

Abstract: Assembly kit, comprising a sonication device (10) and a support (20) for sample tubes (30). The device comprises a tank (13) and an ultrasound wave generator (15) coupled to the tank and designed to generate ultrasound waves having a frequency of between 20 kHz and 80 kHz. The tank comprises an indicator (132) of a level (133) of liquid. The support for sample tubes comprises a means (21) for the support of several sample tubes of a size of 0.2 ml, a rod (22) secured to the support means, and a means (23) for suspending the rod in the tank. The rod has a length such that, in the assembly position, the sample tubes (30) are positioned with their lower end between 8 mm and 12 mm below the indicator (132) of the level of liquid.

Abstract: A method of fragmenting a DNA sequence having a starting size of at least 10000 base pair into fragments having a mean size smaller than or equal to 1300 bp, wherein the DNA sequence is put in a solution, the solution comprising the DNA sequence is put in a container and the container is placed in a liquid bath which is subjected to the action of ultrasound waves such that the ultrasound waves travel through the liquid bath to excite the container and the solution so as to shear the DNA sequence, and wherein the ultrasound waves have a frequency falling in the range between 28 kHz and 80 kHz.

Abstract: A method of fragmenting a DNA sequence having a starting size of at least 10000 base pair into fragments having a mean size smaller than or equal to 1300 bp, wherein the DNA sequence is put in a solution, the solution comprising the DNA sequence is put in a container and the container is placed in a liquid bath which is subjected to the action of ultrasound waves such that the ultrasound waves travel through the liquid bath to excite the container and the solution so as to shear the DNA sequence, and wherein the ultrasound waves have a frequency falling in the range between 28 kHz and 80 kHz.