Patents Assigned to Diagnostics for the Real World, Ltd.
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Patent number: 11524288Abstract: A device for analysing a sample comprising a nucleic acid to be captured and detected using a test strip are described. The device comprises a resilient biasing member disposed in an analysis chamber containing the test strip. The resilient biasing member exerts a force against the test strip sufficient to urge it into the sample chamber when it is in communication with the analysis chamber. This ensures that the test strip is reliably introduced into the sample chamber when it is in communication with the analysis chamber. In one embodiment, the sample chamber comprises guide members for guiding the test strip into the sample chamber. A free end of each guide member is shaped to prevent significant rotation of the test strip, so that the test strip is in correct alignment in the sample chamber for automatic reading of the test result, for example by a camera or optical reader.Type: GrantFiled: December 20, 2018Date of Patent: December 13, 2022Assignee: Diagnostics for the Real World, LTD.Inventors: Philip Stankus, Ivan Hin-Kwan Chew
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Patent number: 10661271Abstract: A device for processing a sample comprises a blister defined by first and second walls. The first wall is flexible allowing the blister to be divided into one or more sealed regions by an external pressure applied to a portion of the first wall. The external pressure is applied in the form of a 2-dimensional shape to form a sealed region having that shape.Type: GrantFiled: June 15, 2017Date of Patent: May 26, 2020Assignee: Diagnostics for the Real World, Ltd.Inventor: Craig Alan Wisniewski
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Patent number: 10330694Abstract: There is provided an automated biological-sample-processing system comprising a pipette, a column of solid-phase material to which nucleic acid binds, a transport apparatus, an air-piston apparatus and an adaptor for coupling the pipette to the transport apparatus and to the air-piston apparatus, in which the adaptor is removably engageable with the transport apparatus and the air-piston apparatus for movement with the transport apparatus during processing of the sample, is couplable to the pipette so that the transport apparatus is controllable to position the pipette and so that the air-piston apparatus is controllable to draw a liquid into the pipette and to expel the liquid from the pipette, and is engageable with the column, in which the adaptor comprises a filter for preventing liquid or aerosol transfer between the pipette or column and the air-piston apparatus.Type: GrantFiled: March 14, 2014Date of Patent: June 25, 2019Assignee: DIAGNOSTICS FOR THE REAL WORLD, LTDInventors: Philip Stankus, Paul Duesbury, Torbjorn Blad, Craig Wisniewski, Jean-Pierre Allain
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Patent number: 10315195Abstract: A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.Type: GrantFiled: October 13, 2017Date of Patent: June 11, 2019Assignees: Diagnostics for the Real World, Ltd., Cambridge Enterprise LimitedInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Craig Alan Wisniewski, Phillip John Stankus
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Patent number: 10184950Abstract: Methods of testing HIV viral load are described. The methods comprise detecting HIV viral RNA in a sample of leukocyte-depleted blood. Such methods can be carried out on low-volume samples obtained without the need for venipuncture or a centrifuge. The methods are particularly suited for HIV viral load testing in resource-limited settings. Methods for monitoring HIV infection are also described, as well as kits for carrying out the methods.Type: GrantFiled: March 14, 2014Date of Patent: January 22, 2019Assignee: DIAGNOSTICS FOR THE REAL WORLD, LTDInventor: Jean-Pierre Allain
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Publication number: 20190018035Abstract: Methods of testing HIV viral load are described. The methods comprise detecting HIV viral RNA in a sample of leukocyte-depleted blood. Such methods can be carried out on low-volume samples obtained without the need for venipuncture or a centrifuge. The methods are particularly suited for HIV viral load testing in resource-limited settings. Methods for monitoring HIV infection are also described, as well as kits for carrying out the methods.Type: ApplicationFiled: September 18, 2018Publication date: January 17, 2019Applicant: DIAGNOSTICS FOR THE REAL WORLD, LTDInventor: Jean-Pierre ALLAIN
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Publication number: 20180008977Abstract: A device for processing a sample comprises a blister defined by first and second walls. The first wall is flexible allowing the blister to be divided into one or more sealed regions by an external pressure applied to a portion of the first wall. The external pressure is applied in the form of a 2-dimensional shape to form a sealed region having that shape.Type: ApplicationFiled: June 15, 2017Publication date: January 11, 2018Applicant: Diagnostics for the Real World, Ltd.Inventor: Craig Alan Wisniewski
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Patent number: 9839909Abstract: A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.Type: GrantFiled: July 27, 2007Date of Patent: December 12, 2017Assignees: Diagnostics for the Real World, Ltd., Cambridge Enterprise LimitedInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Craig Alan Wisniewski, Phillip John Stankus
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Patent number: 9707556Abstract: A device for processing a sample comprises a blister defined by first and second walls. The first wall is flexible allowing the blister to be divided into one or more sealed regions by an external pressure applied to a portion of the first wall. The external pressure is applied in the form of a 2-dimensional shape to form a sealed region having that shape.Type: GrantFiled: August 18, 2008Date of Patent: July 18, 2017Assignee: Diagnostics for the Real World, LTD.Inventor: Craig Wisniewski
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Patent number: 9494584Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: GrantFiled: June 21, 2011Date of Patent: November 15, 2016Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Publication number: 20160024601Abstract: Methods of testing HIV viral load are described. The methods comprise detecting HIV viral RNA in a sample of leukocyte-depleted blood. Such methods can be carried out on low-volume samples obtained without the need for venipuncture or a centrifuge. The methods are particularly suited for HIV viral load testing in resource-limited settings. Methods for monitoring HIV infection are also described, as well as kits for carrying out the methods.Type: ApplicationFiled: March 14, 2014Publication date: January 28, 2016Applicant: DIAGNOSTICS FOR THE REAL WORLD, LTDInventor: Jean-Pierre ALLAIN
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Publication number: 20150099265Abstract: Improved dipstick assays for testing for the presence of a target nucleic acid in a sample solution are described. A dipstick is provided which comprises a contact end for contacting the sample solution and a capture zone remote from the contact end for capturing target nucleic acid. Sample solution is contacted with the contact end to cause sample solution to move by capillary action to the capture zone. Target nucleic acid in the sample solution is captured at the capture zone and is detected by a plurality of different labelled detection probes each capable of hybridizing to a different region of the target nucleic acid. The detection signal is thereby enhanced. In other methods a plurality of different capture probes are added to the sample solution which can then be bound by a capture moiety at the capture zone to indirectly capture target nucleic acid. Capture of target nucleic acid is thereby improved. Kits and dipsticks for carrying out such methods are also described.Type: ApplicationFiled: December 19, 2013Publication date: April 9, 2015Applicant: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva
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Publication number: 20140106347Abstract: Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilised. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilised to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.Type: ApplicationFiled: April 19, 2013Publication date: April 17, 2014Applicant: Diagnostics for the Real World, Ltd.Inventor: Diagnostics for the Real World, Ltd.
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Patent number: 8431336Abstract: Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilized. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilized to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.Type: GrantFiled: July 6, 2001Date of Patent: April 30, 2013Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva, Hsiang Yun Hu
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Publication number: 20120094272Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: ApplicationFiled: June 21, 2011Publication date: April 19, 2012Applicant: DIAGNOSTICS FOR THE REAL WORLD, LTD.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Patent number: 7972837Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: GrantFiled: March 5, 2008Date of Patent: July 5, 2011Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Patent number: 7867706Abstract: Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.Type: GrantFiled: January 19, 2007Date of Patent: January 11, 2011Assignee: Diagnostics For The Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva, Shaun Christopher Hazlewood
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Patent number: 7776617Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: GrantFiled: November 30, 2001Date of Patent: August 17, 2010Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Patent number: 7713746Abstract: The invention provides a dipstick and a kit comprising the dipstick, for testing for the presence of a plurality of different targets in a sample solution which comprises: a dipstick having a plurality of different capture zones and, immobilised to each capture zone, a different capture moiety, each capture moiety capable of capturing a different target; and, separately, a plurality of different detection probes, each detection probe capable of binding to a different target and each detection probe being labelled with or enabling the formation of a detection signal so that the presence of each target is indicated by the formation of a signal at the capture zone for that target; wherein the target for at least two of the capture moieties is a disease causing micro-organism or a marker indicating the existence of a disease, disorder, or condition of the host from which the sample solution was derived, and wherein at least two of the capture moieties are capable of binding to different components or markers of tType: GrantFiled: October 15, 2001Date of Patent: May 11, 2010Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva
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Patent number: 7547298Abstract: In a sampler for sampling a first portion of a liquid flow, the liquid flow enters at a sampler inlet (12). The sample passes through a valve (30) into a sample chamber (8) and then the valve closes, diverting the remainder of the liquid flow to a sampler overflow (6). The valve comprises a valve inlet (24) coupled to the sampler inlet, a valve outlet (38, 36) opening into the sample chamber and positioned, in use, substantially below the valve inlet, a shut-off chamber (32) separating the valve outlet from the valve inlet, and a valve overflow (34) leading from the shut-off chamber to the sampler overflow (6). When the liquid level in the sample chamber rises to a predetermined level it disrupts the flow of liquid through the valve outlet and diverts later flow through the valve inlet into the shut-off chamber and through the valve overflow.Type: GrantFiled: July 25, 2003Date of Patent: June 16, 2009Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Hwai-an Lee, Grant Timothy Lewis Smetham, Phillip John Stankus, Shaun Christopher Hazlewood, Elipidio Cesar Braga Nadala, Craig Alan Wisniewski