Abstract: The invention provides an amplification system for the simultaneous detection of mutant alleles, and identification of the specific mutated sequence. A sample is enriched and simultaneously genotyped by the presence of peptide nucleic acids (PNAs) probes in an homogeneous single tube amplification reaction, by detecting the cleavage of a fluorophore quencher from the 5?-end of PCR products and, concurrently, selecting DNA that includes specific mutations relative to wild type, by employing a thermostable endonuclease that will only cleave an amplicon formed on a mutation bearing template strand. Oligonucleotides and kits for conducting the amplification system are also provided.
Type:
Grant
Filed:
September 24, 2007
Date of Patent:
December 11, 2012
Assignee:
Diasorin S.p.A.
Inventors:
Daniel Adlerstein, Eriet Shehi, Giulia Amicarelli