Abstract: Disclosed is a microfluidic chip capable of accurately and quickly detecting presence of a trace amount of a target within a fluid sample. It has a channel structure comprising a sample inlet for feeding a sample therethrough, a first reservoir for primarily storing the sample therein, a second reservoir for secondarily storing the sample therein, a first reaction portion in which a target is conjugated with a label, a second reaction portion in which the labeled target undergoes a specific reaction, such as an antigen-antibody reaction, and a delaying portion, located between the first and the second reaction portion, for decreasing a flow rate of the sample.

Abstract: Disclosed is a module for rapidly detecting analytes in fluids with high effectiveness and a chip having the module. The module includes a microchannel, which has a filtering zone for removing noise materials and a reaction zone wherein labeling reaction and immobilization reaction for detection of analytes are performed, sample fluid moving through the microchannel due to capillary floating. In a case where the chip having the module is used in detecting analytes in fluids, it is possible to minimize dead volume of sample fluid so that high effective volume ratio can be implemented. Therefore, the chip can be used in detecting analytes from the minimum amount of sample fluid.

Abstract: Disclosed is a method of bonding upper and lower substrates for manufacturing a plastic micro chip comprising the upper substrate, the lower substrate and a sample filling space having a predetermined height for filling a sample between the upper and lower substrates. According to the method, the upper and lower substrates are bonded by introducing an organic solvent between the upper and lower substrates. In addition, the invention provides a method of manufacturing a micro chip using the method and a micro chip manufactured according to the method. According to the invention, it is possible to easily and precisely bond the upper and lower substrates of the plastic micro chip.

Abstract: Disclosed is a fluid analysis chip including a microchannel formed within the chip. The chip includes a sample and a sample outlet communicating with an outside of the chip, and has a structure where the sample inlet and the sample outlet communicate with each other through the closed channel. An expanding part formed in a longitudinal direction of a channel in such a manner that a pair of inner walls corresponding to each other has a larger sectional area at a part or a whole thereof is formed. Therefore, fluids, which pass through the channel adjacent to the expanding part, move while making contact with only another pair of inner walls of the channel, which correspond to each other.

Abstract: Disclosed is a fluid analysis chip including a microchannel formed within the chip. The chip includes a sample and a sample outlet communicating with an outside of the chip, and has a structure where the sample inlet and the sample outlet communicate with each other through the closed channel. An expanding part formed in a longitudinal direction of a channel in such a manner that a pair of inner walls corresponding to each other has a larger sectional area at a part or a whole thereof is formed. Therefore, fluids, which pass through the channel adjacent to the expanding part, move while making contact with only another pair of inner walls of the channel, which correspond to each other.

Abstract: Disclosed is a method of bonding upper and lower substrates for manufacturing a plastic micro chip comprising the upper substrate, the lower substrate and a sample filling space having a predetermined height for filling a sample between the upper and lower substrates. According to the method, the upper and lower substrates are bonded by introducing an organic solvent between the upper and lower substrates. In addition, the invention provides a method of manufacturing a micro chip using the method and a micro chip manufactured according to the method. According to the invention, it is possible to easily and precisely bond the upper and lower substrates of the plastic micro chip.

Abstract: Disclosed is a microchip for a flowcytometer. A channel is expanded around a point, on which laser beams emitted from an optical unit are focused, so that focused sample particles slow down when they pass the expanded portion. This improves the detection intensity of sample particles.

Abstract: A microchannel chip for counting floating microparticles with an optical method is provided. The depth of the microchannel chip is made to be a depth of field such that a clear and sharp optical image of microparticles can be obtained, without necessarily diluting the microparticle mixture inside the microchannel. Consequently, a microparticle counting instrument can easily count microparticles shown on an optical image of the microchannel. Particularly, it enables to count more easily the number of CD3 cells CD4 cells or CD8 cells in white blood cells being stained, without lysing or diluting red blood cells. Therefore, the microchannel chip can advantageously used for counting the number of CD3 cells, CD4 cells or CD8 cells in blood of an AIDS patient to monitor the progression of AIDS.

Abstract: Disclosed is a micro particle analyzing device illuminating light to fluid including micro particles, reading the lights emitted from the micro particles at a signal processing reading section and thus analyzing the micro particles. The device comprises a light source section emitting light which will be illuminated to the fluid; a lens regulating an amount and a focal distance of the light emitted from the light source; and a concave mirror condensing light emitted from the micro particle to reflect it to the reading section wherein the concave mirror is formed with a hole so that the light of the light source section having passed through the lens can pass through the concave mirror. When analyzing the micro particles using the analyzing device according to the invention, the amounts of lights emitted from the micro particles according to up-and-down positions of the micro particles are not different.

Abstract: Disclosed is a focusing channel device which focuses fluid containing micro particles so that the micro particles flow in a line. The channel device comprises a nozzle formed by left and right walls each of which comprises an inclination surface. The cross sectional area in vertical direction decreases from the entrance of the nozzle toward the exit of the nozzle. The shape of cross sectional view in horizontal direction is asymmetric for the central line in the length direction. Using the focus channel device of the invention, the micro particles in the fluid are not combined with each other and passed through the channel one by one. Thus, blockage of the channel or combination and movement of two particles together does not occur.

Abstract: Disclosed is a channel filter for separating microparticles, and more particularly to a channel filter which can easily separate a sample having various sized microparticles by using a surface topology. In the disclosed channel filter, a topology having an upward/downward reference height from a sample inlet to an outlet is continuously or discontinuously formed, and thus it is possible to efficiently separate microparticles from a sample liquid.

Abstract: Disclosed is a a flow cytometer for diagnosis of HIV infections. A fluorescent material having a relatively lower fluorescence intensity is conjugated with one marker and another fluorescent material having a relatively higher fluorescence intensity is conjugated with the other marker. The two markers can be sorted and counted individually and simultaneously from the fluorescence intensity difference.

Abstract: Disclosed is a method for detecting and counting cell surface markers using a difference in fluorescence intensities. A fluorescent material having a relatively lower fluorescence intensity is conjugated with one marker and another fluorescent material having a relatively higher fluorescence intensity is conjugated with the other marker. The two markers can be sorted and counted individually and simultaneously from the fluorescence intensity difference.

Abstract: Disclosed is a focusing channel device which focuses fluid containing micro particles so that the micro particles flow in a line. The channel device comprises a nozzle formed by left and right walls each of which comprises an inclination surface. The cross sectional area in vertical direction decreases from the entrance of the nozzle toward the exit of the nozzle. The shape of cross sectional view in horizontal direction is asymmetric for the central line in the length direction. Using the focus channel device of the invention, the micro particles in the fluid are not combined with each other and passed through the channel one by one. Thus, blockage of the channel or combination and movement of two particles together does not occur.

Abstract: Disclosed is a micro particle analyzing device illuminating light to fluid including micro particles, reading the lights emitted from the micro particles at a signal processing reading section and thus analyzing the micro particles. The device comprises a light source section emitting light which will be illuminated to the fluid; a lens regulating an amount and a focal distance of the light emitted from the light source; and a concave mirror condensing light emitted from the micro particle to reflect it to the reading section wherein the concave mirror is formed with a hole so that the light of the light source section having passed through the lens can pass through the concave mirror. When analyzing the micro particles using the analyzing device according to the invention, the amounts of lights emitted from the micro particles according to up-and-down positions of the micro particles are not different.

Abstract: Disclosed is a microchip for a flowcytometer. A channel is expanded around a point, on which laser beams emitted from an optical unit are focused, so that focused sample particles slow down when they pass the expanded portion. This improves the detection intensity of sample particles.

Abstract: A device for counting micro particles is presented. The device comprises a light source; a chip containing micro particles; an object lens; a CCD camera; a counting part; and a shifter for shifting the position of the chip. It is easy to count the number of micro particles, such as red blood cells or somatic cells, by using the device. The shifter shifts the position of the chip by a predetermined distance at every predetermined time interval in order that a certain area adjacent to the area photographed just before is shifted to the point where the light is incident. Therefore, sub-areas on the chip are photographed successively. The counting part counts the number of micro particles in each sub-area, and adds them together to calculate the total number of micro particles in the samples.

Abstract: An electroporation apparatus comprising an elongated hollow member in order to provide a uniform electric field during electroporation, wherein specifically, electroporation is carried out by applying electric pulses through a couple of electrodes from both end parts of the elongated hollow member, after the hollow member is charged with fluid specimen including cells and material which would be injected into the cells.

Abstract: Disclosed is a method of bonding upper and lower substrates for manufacturing a plastic micro chip comprising the upper substrate, the lower substrate and a sample filling space having a predetermined height for filling a sample between the upper and lower substrates. According to the method, the upper and lower substrates are bonded by introducing an organic solvent between the upper and lower substrates. In addition, the invention provides a method of manufacturing a micro chip using the method and a micro chip manufactured according to the method. According to the invention, it is possible to easily and precisely bond the upper and lower substrates of the plastic micro chip.

Abstract: A method of examining blood type and an apparatus for examining blood type using the method are presented. According to the method, the blood type is examined once the blood is injected into the apparatus, and the result of examination is kept safely. The method comprises introducing the reagents into the plural reagent chambers respectively; flowing the blood into each of reagent chambers and mixing them with the reagents; filtering the mixture of blood; and flowing the mixture of blood which passed the filters-into the reading channels. The introduced blood is passed the micro channels and reacted with the reagents, and the results are obtained from filtering the mixture of blood. Thus, even small quantity of blood may be examined easily and clearly. Further, the results of examination are easily read and kept. The apparatus may be carried out and used with convenience.