Abstract: The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.
Type:
Application
Filed:
July 10, 2001
Publication date:
May 30, 2002
Applicant:
Diosynth RTP, Inc.
Inventors:
Gregory Conn, Brian Reardon, Xianfang Zeng, Chenming Zhang
Abstract: The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.
Type:
Application
Filed:
November 30, 2001
Publication date:
May 9, 2002
Applicant:
Diosynth RTP, Inc.
Inventors:
Gregory Conn, Brian Reardon, Xianfang Zeng, Chenming Zhang