Abstract: Somatic hybrids of Rutaceae plants were obtained by allowing the protoplasts from embryogenic cells of a Rutaceae plant, which are of the nucellar origin, resistant to fusion treatment, and have a high proliferation activity, to fuse with the protoplasts of another Rutaceae plant having no proliferation activity, and then cultivating the fused protoplasts in a medium not containing a phytohormone but containing a saccharide in a concentration suitable for the selective embryogenesis of the hybrid cells.
Type:
Grant
Filed:
October 1, 1985
Date of Patent:
July 10, 1990
Assignees:
Director General of Fruit Tree Research Station Ministry of Agriculture, Forestry and Fisheries, Kikkoman Corporation