Abstract: A process for generating sequence-specific proteases by screening-based directed evolution is disclosed. The use of the process provides proteases recognizing and cleaving user-definable amino-acid sequences with high sequence-specificity. Proteases obtainable by the process can be used in a variety of medical, diagnostic and industrial applications.

Abstract: The present invention provides an insertion, deletion and/or substitution mutein of wild-type Trichoderma reesei ?-mannanase having enhanced thermostability, proteolytic stability, specific activity and/or stability at low pH, a nucleic acid molecule encoding said mannanase mutein, a composition comprising said mannanase mutein; a method for its preparation, and its use for food and feed processing, for coffee extraction and the processing of coffee waste, as a supplement to food and feed, for enzyme aided bleaching of paper pulps, as bleaching and/or desizing agent in textile industry, for oil and gas well stimulation by hydraulic fracturing, as detergent, for removal of biofilms and in delivery systems, or for the processing of renewable resources intended for the production of biological fuels.

Abstract: A method for analyzing properties of a sample by measuring fluorescence parameters in multiple foci, comprises the steps of splitting a collimated primary laser beam with a splitting device into at least two collimated secondary laser beams and deflecting the secondary laser beams such that they propagate at different propagation angles with respect to an optical axis of a focussing optic, focussing the secondary laser beams with the focussing optic into at least two volume elements in the sample, detecting light emitted from the volume elements with a detecting device, and evaluating the detected light for obtaining the properties to be analyzed. Furthermore, a device for implementing this method is described. The invention is particularly suited for high-throughput screening applications.

Abstract: A method for the production of biopolymers with modified properties wherein at least one cycle comprising the following steps is completed: (a) providing a population of single-stranded polynucleotide molecules, wherein individual polynucleotide molecules comprise homologous and heterologous sequence segments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population; (b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments; (c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and (d) temple-directed single-stand synthesizing the degraded ends of the partially degraded double

Abstract: The present invention provides a method for the selective combinatorial randomization (SCR) of polynucleotides at specific sites which comprises providing a double stranded polynucleotide sequence having at least one differing site and selectively randomizing the polynucleotide at or in the proximity to the differing sites without the need for a determination of the sequence position of the differing site.

Abstract: A process for generating sequence-specific proteases by screening-based directed evolution is disclosed. The use of the process provides proteases recognizing and cleaving user-definable amino-acid sequences with high sequence-specificity. Proteases obtainable by the process can be used in a variety of medical, diagnostic and industrial applications.