Abstract: Fluorescence microscopy method comprising: illuminating a scattering sample with a coherent excitation light beam having a wavefront with an initial configuration for exciting fluorescent emitters in the scattering sample; acquiring an initial image; selecting target pixels in an area of the initial image; optimising the initial configuration of the wavefront for each target pixel for decreasing the speckle grain size and obtaining a final image; subtracting the initial image from the final image for each target pixel, obtaining an image; fitting the image with a Gaussian function with free center coordinates for each target pixel; generating an image containing a Gaussian distribution centered at the coordinates and intensity, and with a waist equal to an average size S of speckle grain of the scattering sample, for each target pixel; and generating a final image of the sample by summing the images of the target pixels.
Type:
Grant
Filed:
March 26, 2019
Date of Patent:
January 9, 2024
Assignee:
DISRUPTIVE TECHNOLOGICAL ADVANCES IN LIFE SCIENCE S.R.L.
Inventors:
Marco Leonetti, Giuseppe Antonacci, Raino Ceccarelli
Abstract: Apparatus (1000) and method for automatically detecting odorant substances based on use of nematodes that includes a mechanical selection unit (100) configured to select nematodes in adult stage from an initial nematode population obtaining an intermediate nematode population, a nematode optical selection unit (200) configured to select from the intermediate population a final population of nematodes in adult stage and to select nematodes in young adult stage from nematodes in egg producing adult stage to be sent to a measurement unit (300) configured to detect the response of nematodes of the final population to a stimulus of an odorant substance, the mechanical selection unit (100) being connected to the optical selection unit (200) by a connection channel with an at least three way branch and the optical selection unit (200) being connected to the measurement unit (300) by a loading microchannel.
Type:
Grant
Filed:
October 19, 2018
Date of Patent:
November 29, 2022
Assignees:
FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, DISRUPTIVE TECHNOLOGICAL ADVANCE IN LIFE SCIENCES S.R.L.
Inventors:
Viola Folli, Andrea Santinelli, Marco Broglia