Abstract: A method for killing zebra mussels from a bottom surface of a waterway and from the surfaces of watercraft, docks, pilings and similar facilities located within a waterway. The surfaces to be treated are isolated in a batch of water enclosed within a chamber. The isolated batch of water is thereafter heated to a temperature lethal to the zebra mussels and held at that temperature for a sufficient time to kill the zebra mussels. The isolated batch of water and the treated surface are thereafter released from the chamber and the isolated state.