Abstract: A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
Abstract: A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
Abstract: Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.
Abstract: According to a first aspect of the present invention there is provided a digital signal processing circuit, one or more switches of the circuit being provided by an ion sensitive field effect transistor.
Abstract: A signal processing circuit includes an ion sensitive field effect transistor, a reference electrode for the ion sensitive field effect transistor, a metal oxide semiconductor transistor having its gate coupled to the reference electrode, and a biasing circuit. The biasing circuit is configured to bias the ion sensitive field effect transistor and the metal oxide semiconductor field effect transistor to operate in a weak inversion region and to provide an output current signal.
Abstract: Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.