Abstract: A sample consumable that carries a microvolume of sample to a sample loader. The consumable is precisely aligned utilizing a double-alignment feature to the loader. The loader is based on a crank-slider geometry and allows for simple, one-handed operation for the user. Overall, the consumable and sample loader increase reproducibility of in-line sample loading and offers ease-of-use.

Abstract: An integrated vitals device capable of acquiring multiple data streams, allowing for comprehensive measurement of whole health status using a single, compact device. This allows for simplification of traditional healthcare delivery where multiple devices are required for acquisition of vital signs. A sensor stack up, occupying the approximate physical footprint and volume allows this approach to be possible. This application describes how these simultaneous vitals data streams can be acquired using an integrated device with small mass and volume.

Abstract: A system for collection and delivery of sub-milliliter liquid samples is described that provides a dramatic simplification for the measurement of clinical values. The system produces high dilutions of capillary size samples, such as bodily fluids or blood, by a factor of 1000 or more. Multiple assays can be conducted on a sample of 0.1 cc or less. A small lancet can create a drop of blood on a fingertip or other location, which can be collected into a self-priming capillary blood collector. The sample is then loaded into a consumable loader. Then a fluid, such as saline, is flowed into the consumable loader, and passes through the capillary collector, washing out the fluid. Downstream, the blood in the capillary is reliably diluted by mixing with appropriate reagents to allow analysis of selected blood properties. One or more individual bubbles can be inserted into the stream during loading. The capillary loading system can be used for one or several assays on a single blood sample.

Abstract: The stable and precise incorporation of multiple fluorescent dyes into hydrogel microparticles. The multiple fluorescent dyes, excited with the same source, have distinct fluorescence emission spectra to enable identification of the microparticles. The fluorescent molecules are directly polymerized into the hydrogel matrix or stably incorporated through molecular entanglement. By changing the molar ratios of the fluorescent dyes, different and identifiable microparticles can be synthesized.

Abstract: An integrated fluidics module that reduces mass and volume so that it can readily fit inside a compact biomedical instrument. A fluidics module that integrates discrete components (e.g., pump, connectors, tubing, regulator, and valves) reduces mass and volume requirements.

Abstract: An integrated vitals device capable of acquiring multiple data streams, allowing for comprehensive measurement of whole health status using a single, compact device. This allows for simplification of traditional healthcare delivery where multiple devices are required for acquisition of vital signs. A sensor stack up, occupying the approximate physical footprint and volume allows this approach to be possible. This application describes how these simultaneous vitals data streams can be acquired using an integrated device with small mass and volume.

Abstract: A sample consumable that carries a microvolume of sample to a sample loader. The consumable is precisely aligned utilizing a double-alignment feature to the loader. The loader is based on a crank-slider geometry and allows for simple, one-handed operation for the user. Overall, the consumable and sample loader increase reproducibility of in-line sample loading and offers ease-of-use.

Abstract: An integrated optical block that is highly portable and compact, designed to measure cells and particles.

Abstract: Synthesis of monodisperse PEG-based microparticles with stable coupling chemistries. Biomolecules are conjugated to monodisperse PEG microparticles using non-amine chemistries, such as sulfhydryl groups, azide, or alkyne-based chemistries.

Abstract: Analytical nanostrips for clinical analysis are improved by using multifunctional coding (“multicoding”) to allow simultaneous identification of the particular assay, the value of the assayed analyte, and a calibration of the analyte. The multicoding layout on the nanostrip minimizes the number of zones that are required for a given assay. Moreover, the nanostrip can be scanned in real time during flow of the nanostrip through a detection beam. This both simplifies the assay and allows for alternative means of coding.

Abstract: An improved device and method for passive mixing of fluids is described, and the use of the device in clinical diagnostic procedures. The mixer provides thorough mixing of a sample of blood or other fluid with an assay material, such as a diluent or a component of an assay system, in a closed system with a low and limited pressure drop. Sample size is small, typically 5 to 300 microliters. Mixing is accomplished by a combination of rotational vortex mixing due to a fluid stream coming tangent to a drain, and either or both of a second vortex mixer of opposite handedness, and a Dean mixer. Combinations of these techniques reliably provide complete mixing at low pressure drop. In a preferred usage, the microfluidic system can run a diluent continuously and inject samples at intervals, to facilitate automatic data processing of optical or other signatures of the well-mixed stream.